| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UQCRC2 |
| Uniprot No | P22695 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 15-453aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSLKVAPKVKATAAPAGAPPQPQDLEFTKL PNGLVIASLENYSPVSRIGLFIKAGSRYEDFSNLGTTHLLRLTSSLTTKG ASSFKITRGIEAVGGKLSVTATRENMAYTVECLRGDVDILMEFLLNVTTA PEFRRWEVADLQPQLKIDKAVAFQNPQTHVIENLHAAAYRNALANPLYCP DYRIGKVTSEELHYFVQNHFTSARMALIGLGVSHPVLKQVAEQFLNMRGG LGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVL GAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQ ATAAGDVIKAAYNQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSECFL EEVGSQALVAGSYMPPSTVLQQIDSVANADIINAAKKFVSGQKSMAASGN LGHTPFVDEL |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UQCRC2重组蛋白的参考文献示例(注：文献信息为假设性概括，仅供参考)：
1. **"Structural insights into the human UQCRC2 subunit within mitochondrial complex III"**
*作者：Smith A, et al.*
**摘要**：通过冷冻电镜解析UQCRC2在线粒体复合体III中的三维结构，揭示了其与相邻亚基的相互作用界面，为理解复合体组装及电子传递机制提供依据。
2. **"UQCRC2 mutations impair mitochondrial complex III assembly leading to neurodegenerative disorders"**
*作者：Chen L, et al.*
**摘要**：研究发现UQCRC2基因突变导致复合体III重组蛋白功能缺陷，引发线粒体呼吸链活性下降，与早发性帕金森症相关。
3. **"Recombinant UQCRC2 expression rescues mitochondrial dysfunction in cellular models"**
*作者：Wang X, et al.*
**摘要**：通过重组表达UQCRC2蛋白，成功恢复突变细胞模型中复合体III的酶活性，证实其在维持线粒体能量代谢中的关键作用。
4. **"Functional characterization of UQCRC2 in oxidative phosphorylation"**
*作者：Kimura T, et al.*
**摘要**：利用重组UQCRC2蛋白进行体外功能实验，证明其稳定性影响复合体III的质子泵功能，进而调控细胞ATP合成效率。
(注：以上文献名称及作者为示例性内容，实际研究中请通过学术数据库检索具体文献。)
**Background of UQCRC2 Recombinant Protein**
UQCRC2 (Ubiquinol-Cytochrome c Reductase Core Protein 2) is a nuclear-encoded subunit of mitochondrial Complex III (cytochrome *bc*1 complex), a critical component of the electron transport chain (ETC) responsible for oxidative phosphorylation and ATP production. This 48 kDa protein plays a structural and functional role in stabilizing Complex III and facilitating electron transfer from ubiquinol to cytochrome *c*, coupled with proton translocation across the inner mitochondrial membrane.
Mutations or dysregulation of UQCRC2 are linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s), and cancer, underscoring its importance in cellular energy homeostasis. Recombinant UQCRC2 protein is engineered to study these mechanisms *in vitro*. Typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cell lines), the recombinant protein retains post-translational modifications when expressed in eukaryotic systems, enhancing its functional relevance.
Researchers employ UQCRC2 recombinant proteins to investigate Complex III assembly, ETC dysfunction, and disease-associated mutations. It is also used in drug discovery to screen compounds targeting mitochondrial metabolism or to develop therapies for mitochondrial pathologies. Purification often involves affinity chromatography (e.g., His-tag systems), followed by validation via Western blot or mass spectrometry.
Overall, UQCRC2 recombinant proteins serve as indispensable tools for dissecting mitochondrial biology and advancing translational research in energy-related diseases.
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