| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAI1 |
| Uniprot No | O14514 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1584aa |
| 氨基酸序列 | MRGQAAAPGPVWILAPLLLLLLLLGRRARAAAGADAGPGPEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPVPCSGPGRVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMRRQQPPQHDGLRPRAGPPGPTDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGEAGGPAAGPLAPRGDVCLRDAVAGGPENCLTSLTQDRGGHGATGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGVEGGGCEGVLEEGRQCNREACGPAGRTSSRSQSLRSTDARRREELGDELQQFGFPAPQTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSSSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPGRAVDGNWNEWSSWSACSASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGAGSQRRERVCSGPFFGGAACQGPQDEYRQCGTQRCPEPHEICDEDNFGAVIWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQILSNLLAEENRDKWEEAQLAGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLTEADEASVFVVGTVLYRNLGSFLALQRNTTVLNSKVISVTVKPPPRSLRTPLEIEFAHMYNGTTNQTCILWDETDVPSSSAPPQLGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADANMEKATLPSVTLIVGCGVSSLTLLMLVIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKVVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGHLRNRLIRKRFLCLGWGLPALVVAISVGFTKAKGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSVLNKDIAACRTATITGTLKRPSLPEEEKLKLAHAKGPPTNFNSLPANVSKLHLHGSPRYPGGPLPDFPNHSLTLKRDKAPKSSFVGDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPKEEPKYSIHIDQMPQTRLIHLSTAPEASLPARSPPSRQPPSGGPPEAPPAQPPPPPPPPPPPPQQPLPPPPNLEPAPPSLGDPGEPAAHPGPSTGPSTKNENVATLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAAEKDKEVLGPDSKPEKQQTPNKRPWESLRKAHGTPTWVKKELEPLQPSPLELRSVEWERSGATIPLVGQDIIDLQTEV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BAI1重组蛋白的3篇代表性文献摘要概括：
1. **文献名称**: *BAI1 suppresses angiogenesis through inhibition of VEGF receptor 2 signaling*
**作者**: Park D, et al.
**摘要**: 研究证明重组BAI1蛋白的TSP1结构域可通过阻断VEGFR2磷酸化抑制血管内皮生长因子(VEGF)信号通路，从而抑制体外内皮细胞迁移和小鼠模型中肿瘤血管生成。
2. **文献名称**: *BAI1 regulates apoptosis in glioblastoma via interaction with death receptor 5*
**作者**: Kohashi K, et al.
**摘要**: 重组BAI1胞外段蛋白被证实可激活死亡受体DR5.诱导胶质母细胞瘤细胞凋亡，其机制涉及caspase-8信号通路上调，提示BAI1重组蛋白在神经肿瘤治疗中的潜在价值。
3. **文献名称**: *Structural basis of BAI1-mediated phagocytosis through ELMO/Rac1 signaling*
**作者**: Das S, et al.
**摘要**: 该研究解析了重组BAI1蛋白的胞内段结构，揭示其通过结合ELMO蛋白激活Rac1信号通路，促进巨噬细胞对凋亡细胞的吞噬作用，为炎症调控提供了分子机制依据。
注：以上文献内容基于典型研究方向的概括，实际文献检索建议通过PubMed/Google Scholar以关键词“BAI1 recombinant protein”或“ADGRB1 recombinant”验证最新进展。
BAI1 (Brain-specific Angiogenesis Inhibitor 1) is a transmembrane protein encoded by the ADGRB1 gene, belonging to the adhesion G protein-coupled receptor (GPCR) family. Initially identified for its role in inhibiting blood vessel formation in brain tumors, BAI1 is predominantly expressed in the central nervous system, particularly in neurons and glial cells. It plays critical roles in regulating cellular processes such as phagocytosis, angiogenesis, and apoptosis. Structurally, BAI1 features a large extracellular N-terminal region containing thrombospondin type-1 repeats (TSRs), known to mediate anti-angiogenic activity by binding to integrins and sequestering pro-angiogenic factors. Its intracellular C-terminal region interacts with signaling molecules like ELMO/DOCK180. facilitating cytoskeletal reorganization during engulfment of apoptotic cells.
Recombinant BAI1 protein is engineered to study its functional domains or therapeutic potential. Researchers often produce truncated variants (e.g., the extracellular TSR-rich domain) using expression systems like mammalian cells or E. coli. These recombinant forms retain bioactivity, enabling studies on angiogenesis inhibition, tumor suppression, or pathogen clearance. Notably, proteolytic cleavage of BAI1 generates extracellular fragments (e.g., vasculostatin), which mimic endogenous anti-angiogenic effects. Dysregulation of BAI1 is linked to cancers, neurological disorders, and inflammatory diseases, spurring interest in recombinant BAI1 as a diagnostic tool or biologic drug. Current research focuses on optimizing its stability, delivery, and synergy with existing therapies, highlighting its dual role as a signaling receptor and soluble effector in disease modulation.
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