| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UQCRC1 |
| Uniprot No | P31930 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-480aa |
| 氨基酸序列 | TATFAQ ALQFVPETQV SLLDNGLRVA SEQSSQPTCT VGVWIDVGSR FETEKNNGAG YFLEHLAFKG TKNRPGSALE KEVESMGAHL NAYSTREHTA YYIKALSKDL PKAVELLGDI VQNCSLEDSQ IEKERDVILR EMQENDASMR DVVFNYLHAT AFQGTPLAQA VEGPSENVRK LSRADLTEYL STHYKAPRMV LAAAGGVEHQ QLLDLAQKHL GGIPWTYAED AVPTLTPCRF TGSEIRHRDD ALPFAHVAIA VEGPGWASPD NVALQVANAI IGHYDCTYGG GVHLSSPLAS GAVANKLCQS FQTFSICYAE TGLLGAHFVC DRMKIDDMMF VLQGQWMRLC TSATESEVAR GKNILRNALV SHLDGTTPVC EDIGRSLLTY GRRIPLAEWE SRIAEVDASV VREICSKYIY DQCPAVAGYG PIEQLPDYNR IRSGMFWLRF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与UQCRC1重组蛋白相关的文献示例(注：文献标题与作者为虚构模拟，仅供格式参考)：
1. **标题**: "Structural analysis of recombinant UQCRC1 in mitochondrial complex III assembly"
**作者**: Zhang L, et al.
**摘要**: 本研究通过重组表达人源UQCRC1蛋白，结合冷冻电镜技术解析其在线粒体复合物III中的三维结构，揭示了该亚基在电子传递链组装中的关键相互作用位点。
2. **标题**: "UQCRC1 mutations impair mitochondrial respiration via disrupted cytochrome b-c1 complex stability"
**作者**: Tanaka K, et al.
**摘要**: 通过构建UQCRC1重组蛋白突变体，发现其导致复合物III稳定性下降及ATP合成减少，为线粒体脑肌病提供了分子机制解释。
3. **标题**: "High-yield production of recombinant UQCRC1 in E. coli for antibody generation"
**作者**: Müller R, et al.
**摘要**: 报道了一种优化大肠杆菌表达系统高效生产UQCRC1重组蛋白的方法，并利用该蛋白成功制备了特异性抗体，应用于细胞中线粒体复合物的免疫检测。
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提示：实际文献需通过PubMed/Google Scholar检索关键词 "UQCRC1 recombinant" 或 "UQCRC1 complex III" 获取。真实研究多聚焦于该蛋白在能量代谢、疾病模型(如帕金森症)及复合物组装机制中的作用。
UQCRC1 (Ubiquinol Cytochrome c Reductase Core Protein 1) is a critical subunit of mitochondrial respiratory chain Complex III (cytochrome bc1 complex), a key component of the electron transport chain (ETC) involved in oxidative phosphorylation. Located in the inner mitochondrial membrane, Complex III facilitates electron transfer from ubiquinol to cytochrome c while contributing to the proton gradient across the membrane, which drives ATP synthesis. UQCRC1. as a core structural protein, plays an essential role in maintaining Complex III stability and enzymatic activity. It interacts with other subunits like UQCRC2 and cytochrome b to form the functional holoenzyme.
Recombinant UQCRC1 protein is engineered through heterologous expression systems (e.g., E. coli or mammalian cells) to study its biological functions, structural properties, and interactions. Its production enables research into mitochondrial disorders linked to Complex III dysfunction, such as encephalomyopathy, exercise intolerance, or multisystem diseases caused by UQCRC1 mutations. These mutations may disrupt Complex III assembly, reduce ETC efficiency, and elevate reactive oxygen species (ROS), contributing to cellular stress.
Additionally, recombinant UQCRC1 serves as a tool for investigating metabolic regulation, apoptosis, and pathologies like neurodegeneration, cancer, or cardiovascular diseases. Studies using purified UQCRC1 help dissect its role in mitochondrial dynamics, disease mechanisms, and potential therapeutic targets. Its application extends to antibody development, protein interaction assays, and modeling pathogenic variants to explore genotype-phenotype relationships.
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