| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EWSR1; EWS; RNA-binding protein EWS; EWS oncogene; Ewing sarcoma breakpoint region 1 protein |
| Entrez GeneID | 2130 |
| WB Predicted band size | Calculated MW: 68 kDa; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human EWSR1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于EWSR1抗体的3篇参考文献示例(文献信息为模拟内容，仅供格式参考)：
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1. **文献名称**：*Utility of EWSR1 Antibody in the Diagnosis of Ewing Sarcoma Family Tumors*
**作者**：Smith J, et al.
**摘要**：研究评估了EWSR1抗体在尤文肉瘤家族肿瘤(EFT)中的免疫组化诊断价值，证实其在区分EFT与其他小圆细胞肿瘤中的高敏感性和特异性。
2. **文献名称**：*EWSR1 Rearrangement Detection by FISH and IHC in Soft Tissue Tumors*
**作者**：Lee C, et al.
**摘要**：通过FISH和免疫组化(使用EWSR1抗体)分析软组织肿瘤中EWSR1基因易位，发现抗体染色与分子检测结果高度一致，支持其作为辅助诊断工具。
3. **文献名称**：*EWSR1 Fusion Oncoproteins in Sarcomas: Insights from Immunohistochemical Profiling*
**作者**：Garcia R, et al.
**摘要**：探讨了EWSR1抗体在检测肉瘤中融合蛋白(如EWS-FLI1)的应用，揭示了其在肿瘤分型及靶向治疗研究中的潜在作用。
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注：以上文献为示例，实际引用需通过PubMed、Google Scholar等平台检索真实文献(关键词：EWSR1 antibody, immunohistochemistry, sarcoma)。
The EWSR1 antibody is a crucial diagnostic tool used to detect the presence of the EWSR1 protein, encoded by the *EWSR1* gene located on chromosome 22. This gene is prone to chromosomal translocations, commonly observed in several soft tissue and bone tumors. The EWSR1 protein, part of the TET family of RNA-binding proteins, is involved in transcriptional regulation and RNA processing. However, its oncogenic potential arises when it fuses with genes such as *FLI1* (in Ewing sarcoma) or *ATF1* (in clear cell sarcoma), forming chimeric proteins that drive tumorigenesis.
The EWSR1 antibody is widely employed in immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) to identify tumors with EWSR1 rearrangements. It aids in diagnosing Ewing sarcoma, clear cell sarcoma, desmoplastic small round cell tumor (DSRCT), and other malignancies linked to EWSR1 fusions. Notably, the antibody targets the N-terminal transactivation domain of EWSR1. which is retained in most fusion proteins.
While EWSR1 immunoreactivity supports diagnosis, it is not definitive for specific translocations. Confirmatory molecular testing (e.g., RT-PCR, next-generation sequencing) is required to identify fusion partners. Additionally, some EWSR1-rearranged tumors may show variable antibody reactivity, emphasizing the need for cautious interpretation. Despite limitations, the EWSR1 antibody remains vital in differentiating sarcomas from histologic mimics, guiding targeted therapies, and advancing research into molecular oncogenesis.
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