| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ETS domain-containing protein Elk-1 |
| Entrez GeneID | 2002 |
| WB Predicted band size | Calculated MW: 45 kDa; Observed MW: 62 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human ELK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于ELK1抗体的参考文献示例(均为虚拟文献，实际引用时请核实原文)：
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1. **文献名称**: "ELK1 transcription factor activation by mitogenic signaling in prostate cancer cells"
**作者**: Smith A, et al.
**摘要**: 研究利用ELK1抗体(货号ab123)通过ChIP-seq和Western blot分析发现，ELK1在雄激素受体信号通路中被ERK磷酸化激活，促进前列腺癌细胞增殖，抗体特异性通过siRNA敲低验证。
2. **文献名称**: "Role of ELK1 in neuronal differentiation: Insights from CRISPR/Cas9 and antibody-based assays"
**作者**: Chen L, et al.
**摘要**: 通过ELK1抗体(克隆号2B2)的免疫荧光和流式细胞术，证实ELK1在小鼠神经干细胞分化中调控Sox9基因表达，抗体特异性在ELK1敲除细胞系中验证无交叉反应。
3. **文献名称**: "ELK1-mediated regulation of inflammatory genes in macrophage polarization"
**作者**: Gupta R, et al.
**摘要**: 使用ELK1抗体(品牌CellSignaling #9876)进行染色质免疫共沉淀(ChIP)和ELISA，发现ELK1与NF-κB协同激活炎症因子IL-6和TNF-α，抗体适用于人/鼠样本。
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注：以上文献信息为模拟生成，实际研究中建议通过PubMed或Web of Science检索关键词“ELK1 antibody”、“ELK1 ChIP”等获取真实文献。
The ELK1 antibody is a crucial tool in studying the ELK1 protein, a member of the ETS-domain transcription factor family. ELK1 plays a pivotal role in regulating gene expression by binding to specific DNA sequences, often in conjunction with serum response factor (SRF), to activate transcription of target genes involved in cell proliferation, differentiation, and apoptosis. It is a downstream effector of the mitogen-activated protein kinase (MAPK) signaling pathway, where phosphorylation by kinases like ERK modulates its transcriptional activity.
ELK1 contains a conserved ETS DNA-binding domain and a transactivation domain, enabling its interaction with co-regulators and chromatin. Researchers use ELK1 antibodies to detect and quantify ELK1 expression, localization, and post-translational modifications in various experimental settings, including Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence. These antibodies are essential for investigating ELK1's role in cellular responses to extracellular signals, stress, and developmental cues.
Dysregulation of ELK1 has been linked to cancers, neurological disorders, and cardiovascular diseases, making its study clinically relevant. ELK1 antibodies are typically validated for specificity across species (human, mouse, rat) and tested for cross-reactivity with related ETS proteins. Both monoclonal and polyclonal variants are available, with selection depending on application requirements. Their use has advanced understanding of transcriptional networks in health and disease, highlighting ELK1 as a potential therapeutic target.
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