首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TRACP-5b含量。TRACP-5b是一种靶点。它与骨代谢紧密相关，在破骨细胞中高度表达。研究机制方面，主要聚焦于其在破骨细胞活化、骨吸收过程中的作用。检测其水平变化，有助于评估骨代谢状态和相关疾病如骨质疏松等的发病机制。试剂盒检测范围为0.312 mIU/mL-20 mIU/mL，在骨质疏松机制研究、骨骼疾病模型评估及骨代谢相关药物开发等领域具有重要科研价值；适用于体外定量分析骨代谢相关动物模型的生物标志物表达水平，为骨生物学研究、药物干预效果评价及病理机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TRACP-5b
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 mIU/mL-20 mIU/mL
灵敏度：0.078 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-102 | |||||
回收率：
| The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-96 | ||||
| EDTA plasma (n=4) | 95 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected202.0761.9882.0321.887101.2921.1961.2441.09950.8040.8200.8120.6672.50.5110.5210.5160.3711.250.3240.3350.3300.1850.6250.2820.2780.2800.1350.3120.2230.2120.2180.07300.1460.1440.145 | ||||||
货期：3-5 working days
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