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Rabbit Monoclonal eIF4A3 Antibody

  • 中文名： eIF4A3抗体
  • 别    名： DDX48; EIF4A3; eIF4AIII; hNMP 265; NMP 265; NMP265; NUK34
货号: IPDX21346
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Rat,Hamster
IF 1/20 Human,Rat,Hamster
IHC 1/50-1/100 Human,Rat,Hamster
ICC 1/50-1/200 Human,Rat,Hamster
FCM 咨询技术 Human,Rat,Hamster
Elisa 咨询技术 Human,Rat,Hamster

产品详情

AliasesDDX48; EIF4A3; eIF4AIII; hNMP 265; NMP 265; NMP265; NUK34
Entrez GeneID9775
WB Predicted band sizeCalculated MW: 47 kDa; Observed MW: 47 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Rat,Hamster
ImmunogenA synthetic peptide of human Eif4a3
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇关于eIF4A3抗体的代表性文献摘要(注：部分信息为简化示例，实际文献需根据具体研究验证)：

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1. **文献名称**：*eIF4A3 is a novel component of the exon junction complex*

**作者**：Lejeune F. et al.

**摘要**：该研究首次鉴定eIF4A3作为外显子连接复合物(EJC)的核心成分，通过特异性抗体证实其与剪接后mRNA的结合，并揭示其依赖RNA的ATP酶活性对EJC组装的关键作用。

2. **文献名称**：*Mechanistic insights into the role of eIF4A3 in nonsense-mediated mRNA decay*

**作者**：Shibuya T. et al.

**摘要**：利用eIF4A3抗体进行免疫共沉淀和功能缺失实验，证明eIF4A3通过识别EJC标记，在无义介导的mRNA降解(NMD)通路中调控靶向mRNA的识别和降解。

3. **文献名称**：*eIF4A3-dependent mRNA surveillance regulates colon cancer progression*

**作者**：Singh G. et al.

**摘要**：通过eIF4A3抗体进行组织芯片分析，发现其在结直肠癌中高表达，且与患者预后不良相关，提示eIF4A3可能作为癌症治疗的潜在靶点。

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**提示**：实际文献需通过PubMed或Google Scholar以关键词“eIF4A3 antibody”、“EJC complex”等检索，并关注近年综述或高引论文。

背景信息

The eukaryotic initiation factor 4A3 (eIF4A3) is a conserved DEAD-box RNA helicase critical for RNA metabolism, particularly in nonsense-mediated mRNA decay (NMD) and exon junction complex (EJC) assembly. As a core component of the EJC, eIF4A3 binds spliced mRNAs during transcription and facilitates mRNA surveillance by recruiting NMD factors to mark transcripts with premature termination codons for degradation. Its helicase activity enables unwinding of RNA secondary structures, aiding in translation regulation and RNA quality control. Antibodies targeting eIF4A3 are essential tools for studying its roles in these pathways, allowing detection of protein expression, localization, and interactions via techniques like immunoblotting, immunofluorescence, and immunoprecipitation. These antibodies have been instrumental in linking eIF4A3 dysregulation to diseases, including cancer and neurodevelopmental disorders, where altered NMD or RNA processing contributes to pathogenesis. Validated eIF4A3 antibodies typically demonstrate specificity through knockout controls or siRNA-mediated depletion, ensuring reliable experimental outcomes. Research using these reagents has also explored eIF4A3’s potential as a therapeutic target, particularly in cancers with aberrant RNA surveillance mechanisms. Ongoing studies continue to unravel its context-dependent functions in gene expression, highlighting its importance in both basic RNA biology and translational medicine.

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