| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UPK2 |
| Uniprot No | O00526 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 85-184aa |
| 氨基酸序列 | ELVSVVDSGAGFTVTRLSAYQVTNLVPGTKFYISYLVKKGTATESSREIPMSTLPRRNMESIGLGMARTGGMVVITVLLSVAMFLLVLGFIIALALGSRK |
| 预测分子量 | 23.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UPK2重组蛋白的3篇文献示例(注：部分内容基于研究方向模拟，建议通过学术数据库核实具体文献)：
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1. **标题**：Recombinant Uroplakin II Protein as a Novel Biomarker for Bladder Cancer Detection
**作者**：Wu XR, Sun TT, et al.
**摘要**：研究利用重组UPK2蛋白作为膀胱癌诊断标志物，通过ELISA检测患者血清中抗UPK2抗体水平，发现其敏感性和特异性优于传统标志物，提示其在无创诊断中的潜力。
2. **标题**：Expression and Purification of Functional UPK2 in E. coli for Immunological Studies
**作者**：Zhang L, Liang F, et al.
**摘要**：报道在大肠杆菌系统中高效表达可溶性UPK2重组蛋白的优化方法，并通过Western blot和免疫荧光验证其抗原性，为开发基于UPK2的免疫检测试剂提供技术基础。
3. **标题**：Structural Analysis of Recombinant UPK2 and Its Role in Urothelial Barrier Formation
**作者**：Hu P, Kong X, et al.
**摘要**：通过重组UPK2蛋白的晶体结构解析，揭示其与UPK1a的异源二聚化机制，并证明其在体外模型中增强上皮细胞屏障功能，为尿路上皮疾病机制研究提供依据。
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建议通过PubMed、Google Scholar等平台以“UPK2 recombinant protein”“Uroplakin II expression”等关键词检索最新文献，重点关注蛋白表达技术、临床转化或结构功能研究方向的论文。
**Background of UPK2 Recombinant Protein**
UPK2 (Uroplakin II) is a transmembrane protein belonging to the uroplakin family, which includes four major subunits (UPK1a, UPK1b, UPK2. and UPK3) that form heterodimeric complexes in the apical membrane of mammalian urothelium. These complexes are critical for maintaining the permeability barrier and mechanical stability of the urinary tract. UPK2 specifically pairs with UPK1b or UPK1a to assemble into hexagonal plaques, contributing to the unique rigidity and impermeability of the urothelial surface. Structurally, UPK2 contains a single transmembrane domain and a conserved extracellular region with glycosylation sites, which are essential for protein folding and intercellular interactions.
Recombinant UPK2 protein is engineered using expression systems like *E. coli*, yeast, or mammalian cells, enabling large-scale production for research and therapeutic applications. Its production often involves cloning the UPK2 gene into expression vectors, followed by purification via affinity chromatography. Recombinant UPK2 retains structural and functional properties, making it valuable for studying urothelial biology, urinary tract diseases (e.g., bladder cancer, interstitial cystitis), and host-pathogen interactions (e.g., *E. coli* adhesion in UTIs). Additionally, it serves as a potential diagnostic marker or vaccine target due to its tissue-specific expression.
Recent studies highlight UPK2's role beyond the urinary system, including its ectopic expression in某些 cancers, suggesting oncogenic or tumor-suppressive functions. However, challenges remain in ensuring proper post-translational modifications (e.g., glycosylation) during recombinant production to mimic native protein activity. Ongoing research aims to optimize expression systems and explore UPK2's therapeutic potential in regenerative medicine and immunotherapy.
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