| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDA02; EIF-2A; MST089; MSTP004; MSTP089 |
| Entrez GeneID | 83939 |
| WB Predicted band size | Calculated MW: 65 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human eIF2A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于eIF2A抗体的3篇参考文献示例(文献信息为虚构，仅供示例参考)：
1. **"eIF2A mediates selective translation of viral mRNAs under stress conditions"**
*作者：Starck, S.R., et al.*
摘要：研究揭示了eIF2A在哺乳动物细胞中特异性调控病毒mRNA翻译的作用，通过使用特异性eIF2A抗体，证实其在应激条件下替代eIF2参与特定mRNA的起始过程。
2. **"Functional characterization of eIF2A knockout models using isoform-specific antibodies"**
*作者：Sendoel, A., et al.*
摘要：通过开发高特异性eIF2A抗体，验证了eIF2A敲除细胞系中蛋白表达的缺失，并证明eIF2A在特定生理条件下(如氨基酸饥饿)调控内部核糖体进入位点(IRES)介导的翻译。
3. **"eIF2A expression correlates with tumor progression in hepatocellular carcinoma"**
*作者：Wang, L., et al.*
摘要：利用商业化eIF2A抗体进行免疫组化分析，发现eIF2A在肝癌组织中高表达，且其水平与患者预后不良相关，提示其作为潜在癌症标志物的可能性。
(注：以上文献为生成示例，实际研究中请通过PubMed或学术数据库检索真实发表的论文。)
The eukaryotic initiation factor 2A (eIF2A) is a lesser-studied protein involved in the regulation of translation initiation under stress conditions. While the canonical eIF2 complex (eIF2α/β/γ) is central to global protein synthesis, eIF2A has been proposed to mediate alternative translation pathways, particularly when eIF2α is phosphorylated during cellular stress (e.g., viral infection, nutrient deprivation). eIF2A may facilitate the selective translation of specific mRNAs, such as those containing internal ribosome entry sites (IRES), including stress-responsive transcripts like ATF4. However, its precise mechanisms and physiological relevance remain debated due to conflicting studies and limited characterization.
Anti-eIF2A antibodies are critical tools for investigating its expression, localization, and interactions. Commercial antibodies are typically developed using immunogens derived from conserved regions of human or mouse eIF2A. They are validated in applications such as Western blotting, immunoprecipitation, and immunofluorescence. Researchers employ these antibodies to explore eIF2A's role in stress granule formation, viral replication, and diseases like cancer or neurodegeneration. Challenges include cross-reactivity concerns due to sequence similarities with other eIF family members, necessitating careful validation using knockout controls. Current studies focus on clarifying whether eIF2A acts as a backup initiation factor or a specialized regulator in stress adaptation, making its antibody a key reagent in translational control research.
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