首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠翻译调节肿瘤蛋白(TPT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TPT1含量。TPT1 即硫氧还蛋白结合蛋白 1，目前它在肿瘤等疾病领域是备受关注的研究靶点。其参与细胞内氧化还原平衡的调节，可能与肿瘤细胞的增殖、存活及转移相关，通过研究其作用机制，有望为相关疾病的诊断和治疗提供新方向。试剂盒检测范围为9.37 pg/mL-600 pg/mL，本试剂盒适用于科研领域中对小鼠模型或体外培养细胞中TPT1蛋白的定量分析，例如肿瘤相关信号通路研究、药物干预效果评估或疾病模型中蛋白动态变化的检测，为探索TPT1在疾病发生发展中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tpt1 ELISA Kit; Trt ELISA Kit; Translationally-controlled tumor protein ELISA Kit; TCTP ELISA Kit; 21 kDa polypeptide ELISA Kit; p21 ELISA Kit; p23 ELISA Kit
缩写：TPT1
Uniprot No.：P63028
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：9.37 pg/mL-600 pg/mL
灵敏度：2.34 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 86-98 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 90 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected6002.8362.6722.7542.6333002.0992.1842.1422.0211501.4941.4831.4891.368750.8220.7650.7940.67337.50.4980.4610.4800.35918.750.2670.2460.2570.1369.370.1860.1780.1820.06100.1250.1170.121 | ||||||
货期：3-5 working days
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