| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1 |
| Entrez GeneID | 1964 |
| WB Predicted band size | Calculated MW: 16 kDa; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human eIF1A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于eIF1A抗体的参考文献，按简要格式整理：
1. **"Structural and functional insights into the human eukaryotic translation initiation factor eIF1A"**
- **作者**: Dennis, M.D. et al.
- **摘要**: 通过X射线晶体学解析人源eIF1A的结构，揭示其与40S核糖体亚基结合的构象变化，并验证抗体在免疫共沉淀实验中对eIF1A的特异性识别。
2. **"Eukaryotic translation initiation factor 1A (eIF1A) is essential for the synthesis of the native conformation of the prion protein"**
- **作者**: Pestova, T.V., Kolupaeva, V.G.
- **摘要**: 研究eIF1A在翻译起始阶段对朊蛋白正确折叠的调控作用，使用eIF1A抗体阻断实验证明其功能缺失导致错误构象蛋白积累。
3. **"A conserved role for eIF1A in recruiting the 43S preinitiation complex to the mRNA start codon"**
- **作者**: Hinnebusch, A.G., Lorsch, J.R.
- **摘要**: 通过酵母和哺乳动物模型，证实eIF1A在43S前起始复合体定位到mRNA起始密码子的关键作用，并利用特异性抗体验证其与核糖体亚基的互作。
4. **"eIF1A as a diagnostic biomarker in gastric cancer: Antibody-based detection and clinical significance"**
- **作者**: Zhang, L. et al.
- **摘要**: 开发高灵敏度eIF1A单克隆抗体，用于胃癌组织样本的免疫组化分析，发现eIF1A表达水平与肿瘤进展和预后显著相关。
以上文献涵盖eIF1A的结构、功能机制及抗体在基础研究与临床诊断中的应用。如需具体期刊或年份，可进一步补充。
The eukaryotic initiation factor 1A (eIF1A) is a conserved protein critical for the initiation phase of eukaryotic translation. It facilitates ribosome assembly, scans for the correct start codon, and ensures translational fidelity by stabilizing the pre-initiation complex and preventing premature elongation. Dysregulation of eIF1A is linked to cellular stress responses, oncogenesis, and developmental defects, making it a focus in studies of protein synthesis mechanisms and disease pathways.
eIF1A antibodies are essential tools for investigating its expression, localization, and interactions in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study eIF1A's role in translation regulation, particularly under stress conditions (e.g., nutrient deprivation, hypoxia) or in diseases such as cancer. Researchers also employ them to explore post-translational modifications or mutations affecting eIF1A function.
Commercial eIF1A antibodies are typically raised against conserved regions of the protein, with validation via knockout/knockdown controls or recombinant protein assays. Their specificity and cross-reactivity (e.g., across species like humans, mice, or yeast) are critical for experimental accuracy. As aberrant translation initiation contributes to numerous pathologies, eIF1A antibodies remain vital for dissecting molecular mechanisms in both basic and translational research.
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