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产品描述：人翻译调节肿瘤蛋白(TPT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TPT1含量。TPT1靶点在生命活动中扮演一定角色。其相关研究不断深入，机制方面主要围绕在细胞内参与多种生理过程，如调节细胞增殖、凋亡等，对其深入研究有望为相关疾病的发病机理、诊断及治疗提供新的方向。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于基础科研中探索TPT1在肿瘤生物学、细胞应激反应或药物干预下的表达调控机制，也可用于体外模型中分析该蛋白在不同病理生理条件下的动态变化，助力于癌症相关分子机制研究及生物标志物筛选。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ27337 ELISA Kit; Fortilin ELISA Kit; Histamine releasing factor ELISA Kit; Histamine-releasing factor ELISA Kit; HRF ELISA Kit; Immunoglobulin E-dependent ELISA Kit; p02 ELISA Kit; p23 ELISA Kit; TCTP ELISA Kit; TCTP_HUMAN ELISA Kit; tpt1 ELISA Kit; Translationally controlled tumor protein ELISA Kit; Translationally-controlled tumor protein ELISA Kit; Tumor protein translationally controlled 1 ELISA Kit; tumor protein; translationally-controlled 1 ELISA Kit
缩写：TPT1
Uniprot No.：P13693
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of human TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 97-107 | ||||
| EDTA plasma (n=4) | 92 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.3522.2362.2942.1614001.7991.7771.7881.6552001.2181.2551.2371.1041000.6790.7090.6940.561500.4540.4800.4670.334250.2450.2360.2410.10812.50.2020.2050.2040.07100.1270.1380.133 | ||||||
货期：3-5 working days
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