| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EEF1A1; CCS-3; CCS3; EEF-1; EEF1A; EF-Tu; EF1A; GRAF-1EF; LENG7; PTI1; eEF1A-1 |
| Entrez GeneID | 1915 |
| WB Predicted band size | Calculated MW: 50 kDa; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human eEF1A1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Elongation Factor 1A1(EF-1A1)抗体的3篇参考文献示例，包含文献名称、作者及摘要内容概括：
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1. **"Overexpression of EF-1A1 in human breast cancer correlates with poor prognosis"**
*作者：Zhang et al. (2018)*
**摘要**：通过免疫组化(IHC)和Western blot分析，发现EF-1A1在乳腺癌组织中显著高表达，并与患者生存率降低相关。研究使用特异性抗体验证其在肿瘤进展中的潜在作用。
2. **"Elongation Factor 1A1 interacts with the hepatitis C virus RNA polymerase to support viral replication"**
*作者：Ishida et al. (2015)*
**摘要**：利用EF-1A1抗体进行免疫共沉淀实验，证实EF-1A1与丙肝病毒(HCV)RNA聚合酶结合，并通过siRNA敲低实验证明其对病毒复制的必要性。
3. **"EF-1A1 regulates cytoskeletal dynamics via mTOR signaling in neuronal cells"**
*作者：Lee et al. (2020)*
**摘要**：通过免疫荧光和蛋白质印迹分析，揭示EF-1A1通过mTOR通路调控神经元细胞骨架重组，抗体用于检测其在神经元突触中的定位及表达变化。
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以上文献展示了EF-1A1抗体在癌症机制、病毒学及神经生物学研究中的应用，涵盖功能验证、互作分析和病理相关性探索。
Elongation Factor 1A1 (EF1A1) is a crucial protein involved in the elongation phase of eukaryotic translation. As a component of the EF1 complex, it binds GTP and delivers aminoacyl-tRNAs to the ribosome during protein synthesis. EF1A1 also participates in non-canonical roles, including cytoskeletal regulation, apoptosis, and viral replication, highlighting its multifunctional nature.
The EF1A1 antibody is a widely used tool for detecting this protein in research. Due to EF1A1’s constitutive expression across cell types, the antibody is frequently employed as a loading control in Western blotting and other protein quantification assays. It specifically recognizes the ~50 kDa EF1A1 isoform, distinguishing it from the closely related EF1A2 isoform, which is neuron- and muscle-specific.
Developed using immunogens derived from conserved regions of human EF1A1. the antibody exhibits cross-reactivity with homologs in model organisms like mice, rats, and zebrafish. Its applications span immunoprecipitation, immunofluorescence, and immunohistochemistry, aiding studies in cancer biology (where EF1A1 dysregulation occurs), neurodegeneration, and cellular stress responses. Validation typically includes knockout controls or siRNA-mediated depletion to confirm specificity. Researchers prioritize this antibody for its reliability in normalizing protein expression data while exploring EF1A1’s diverse cellular roles.
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