| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E2F4; Transcription factor E2F4; E2F-4 |
| Entrez GeneID | 1874 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human E2F4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于E2F4抗体的3篇参考文献示例(内容基于模拟生成，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Characterization of a Novel Monoclonal Antibody Specific to E2F4 and Its Application in Cancer Research*"
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种新型单克隆抗体，特异性靶向人E2F4蛋白。通过Western blot和免疫组化验证，该抗体在多种癌细胞系中检测到E2F4的表达，并揭示了其在肿瘤细胞周期调控中的作用。
2. **文献名称**: *"E2F4 Dysregulation in Senescence: Insights from Antibody-Based Chromatin Immunoprecipitation*"
**作者**: Lee H, et al.
**摘要**: 利用高特异性E2F4抗体进行染色质免疫沉淀(ChIP)，研究发现E2F4在细胞衰老过程中结合特定靶基因启动子，调控p53依赖性细胞周期停滞，提示其作为潜在衰老标志物。
3. **文献名称**: *"Comparative Analysis of E2F Family Antibodies in Pancreatic Tumor Samples*"
**作者**: Garcia R, et al.
**摘要**: 该研究比较了多种市售E2F家族抗体(包括E2F4)的特异性，发现部分抗体存在交叉反应。通过CRISPR敲除验证，确认了某品牌E2F4抗体在胰腺癌组织中的可靠性，为临床样本分析提供参考。
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如需获取真实文献，建议在 **PubMed** 或 **Google Scholar** 中检索关键词“E2F4 antibody”、“E2F4 antibody validation”或结合研究领域(如“cancer”“cell cycle”)。
The E2F4 antibody is a crucial tool in molecular and cancer biology research, primarily used to detect and study the E2F4 protein, a member of the E2F transcription factor family. E2F4 plays a dual role in regulating cell cycle progression and differentiation. Unlike other E2F members that promote cell cycle entry (e.g., E2F1-3), E2F4 primarily functions as a transcriptional repressor, often partnering with retinoblastoma (pRb) family proteins (p130 and p107) to form inhibitory complexes. These complexes suppress cell cycle-related genes, maintaining quiescence or driving terminal differentiation in specific cell types. Dysregulation of E2F4 is implicated in cancers, where its aberrant expression may contribute to tumor progression or suppression depending on context.
E2F4 antibodies are widely employed in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and DNA-binding activity. Researchers use these antibodies to explore E2F4's interactions with partner proteins and its role in cellular responses to oncogenic signals or therapeutic agents. Validated E2F4 antibodies are essential for specificity, as cross-reactivity with other E2F family members can confound results. Many commercial antibodies are raised against conserved epitopes, enabling cross-species reactivity (e.g., human, mouse, rat). Recent studies also utilize E2F4 antibodies to profile its occupancy across the genome, revealing context-dependent regulatory networks in development and disease.
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