首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肥大细胞类胰蛋白酶(MCT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的TPSAB1含量。TPSAB1，也称为类胰蛋白酶α/β 1，是一种胰蛋白酶样丝氨酸蛋白酶，属于肽酶家族S1。它在肝素稳定的四聚体中具有酶促活性，对内源性蛋白酶抑制剂有抗性。TPSAB1基因位于染色体16p13.3，具有高度保守的3' UTR和串联重复序列，其等位基因表现出序列变异，被认为是哮喘和其他过敏性和炎症性疾病的发病机制中的介质。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础科研中肥大细胞相关机制的探索，如药物干预实验、疾病模型评估或炎症因子网络研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tpsab1 ELISA Kit; Mcp7 ELISA Kit; Mcpt7 ELISA Kit; Tpsb1 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; rMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit; Tryptase ELISA Kit; skin ELISA Kit
缩写：TPSAB1
Uniprot No.：P27435
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, urine
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 90-92 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of rat MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 104 | 100-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.4642.4892.4772.326201.8231.8631.8431.692101.2291.2631.2461.09550.8120.8470.8300.6792.50.4820.4980.4900.3391.250.3120.3390.3260.1750.6250.2410.2580.2500.09900.1500.1520.151 | ||||||
货期：3-5 working days
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