Rat Thyroid-Peroxidase,TPO ELISA Kit

货号: IPD-E08352r

Price： ￥2500

48T 96T

数量：

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产品描述：大鼠甲状腺过氧化物酶(TPO)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TPO含量。TPO即血小板生成素，其作为造血调控因子，在血小板生成过程中有关键作用。研究机制主要围绕它结合并激活跨膜TPO受体，启动信号传导途径，促进造血干细胞向巨核系祖细胞分化、增殖及成熟，进而产生血小板。试剂盒检测范围为2.5 ng/mL-40ng/mL，适用于甲状腺相关疾病的动物模型研究、环境毒理评估中甲状腺功能干扰物的筛选，以及甲状腺自身抗体作用机制等基础科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
缩写：TPO
Uniprot No.：P14650
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue Homogenates
检测范围：2.5 ng/mL-40ng/mL
灵敏度：2.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-97
1:2	Average %	95
	Range %	84-99
1:4	Average %	90
	Range %	82-100
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rat TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-102
EDTA plasma (n=4)	94	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2Average　400.1600.1540.157　200.2690.2650.267　100.4990.5120.506　51.0431.0071.025　2.52.0512.0372.044

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