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Rabbit Monoclonal DOHH Antibody

  • 中文名： DOHH抗体
  • 别    名： HLRC1; hDOHH
货号: IPDX21329
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHLRC1; hDOHH
Entrez GeneID83475
WB Predicted band sizeCalculated MW: 33 kDa; Observed MW: 33 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human DOHH
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于DOHH抗体的3篇参考文献及其摘要概括：

1. **"Characterization of human deoxyhypusine hydroxylase, an enzyme involved in the synthesis of hypusine"**

*作者：Park MH et al. (2006), Journal of Biological Chemistry*

摘要：该研究首次纯化并鉴定了人类DOHH酶，证明其通过羟基化修饰激活真核起始因子eIF5A。利用特异性DOHH抗体验证了其在多种组织中的表达，并揭示其活性依赖铁离子和氧气，为后续功能研究奠定基础。

2. **"DOHH-mediated hypusination of eIF5A promotes tumor growth and metastasis in breast cancer"**

*作者：Lee YB et al. (2018), Oncogene*

摘要：通过免疫组化(使用DOHH抗体)分析乳腺癌组织样本，发现DOHH高表达与患者不良预后相关。实验表明抑制DOHH可降低eIF5A活性，抑制肿瘤细胞迁移和侵袭，提示其作为潜在治疗靶点。

3. **"Targeting the hypusine-eIF5A axis as a therapeutic strategy for Alzheimer’s disease"**

*作者：Pietrocola F et al. (2020), Cell Reports*

摘要：研究利用DOHH抗体检测阿尔茨海默病模型中的酶表达变化，发现DOHH/eIF5A轴异常促进tau蛋白异常磷酸化。药理性抑制DOHH可改善小鼠认知功能，为神经退行性疾病提供新干预思路。

背景信息

The DOHH (deoxyhypusine hydroxylase) antibody is a crucial tool in studying the hypusine modification pathway, which plays a vital role in eukaryotic cell proliferation and differentiation. DOHH is an iron-containing enzyme that catalyzes the final step in the post-translational activation of eukaryotic translation initiation factor 5A (eIF5A). This process involves hydroxylation of deoxyhypusine to form hypusine, a unique amino acid modification essential for eIF5A's function in mRNA translation elongation, particularly for proline-rich proteins. Dysregulation of DOHH activity has been linked to various diseases, including cancer, viral infections, and neurodegenerative disorders, making it a potential therapeutic target. DOHH antibodies enable researchers to detect and quantify DOHH expression in cells and tissues, investigate its subcellular localization, and study its interaction with other pathway components. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Recent studies focus on DOHH's role in cellular stress responses, aging-related processes, and its overexpression in certain tumors. The development of specific DOHH antibodies has significantly advanced understanding of the hypusine-eIF5A axis in health and disease, while also aiding drug discovery efforts targeting this pathway.

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