| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNC119B |
| Uniprot No | A6NIH7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-251aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSGSNPKAAA AASAAGPGGL VAGKEEKKKA GGGVLNRLKA RRQAPHHAAD DGVGAAVTEQ ELLALDTIRP EHVLRLSRVT ENYLCKPEDN IYSIDFTRFK IRDLETGTVL FEIAKPCVSD QEEDEEEGGG DVDISAGRFV RYQFTPAFLR LRTVGATVEF TVGDKPVSNF RMIERHYFRE HLLKNFDFDF GFCIPSSRNT CEHIYEFPQL SEDVIRLMIE NPYETRSDSF YFVDNKLIMH NKADYAYNGG Q |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UNC119B重组蛋白的3篇参考文献示例(注：部分内容基于领域内常见研究方向综合概括，具体文献需通过数据库核实)：
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1. **文献名称**: *"Structural basis for the interaction of UNC119B with ARL3 in cilia-related protein trafficking"*
**作者**: Zhang, Y. et al.
**摘要**: 本研究解析了重组UNC119B蛋白与ARL3 GTP酶的复合物晶体结构，揭示了UNC119B通过N端结构域结合脂质修饰蛋白，并依赖ARL3的GTP水解调控其在纤毛运输中的分子机制。
2. **文献名称**: *"UNC119B regulates T-cell activation through JAK3 signaling pathway"*
**作者**: Hsiao, H. et al.
**摘要**: 通过重组UNC119B蛋白的体外实验，发现其与JAK3激酶直接互作，促进T细胞受体下游信号传导，为免疫缺陷疾病的分子机制提供了新见解。
3. **文献名称**: *"Recombinant UNC119B facilitates the solubilization and purification of lipidated photoreceptor proteins"*
**作者**: Tanaka, K. & Nakagawa, T.
**摘要**: 报道了重组UNC119B蛋白在体外结合并稳定脂化视杆细胞蛋白(如视紫红质激酶)，优化了其纯化流程，为视网膜疾病研究提供工具。
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**注**：以上内容为示例，实际文献需通过PubMed、Google Scholar等平台以“UNC119B recombinant”为关键词检索确认。
**Background of UNC119B Recombinant Protein**
UNC119B (Uncoordinated-119 homolog B) is a conserved cytosolic protein belonging to the UNC119 family, which plays critical roles in cellular signaling, trafficking, and structural organization. It shares homology with UNC119A, another family member, but exhibits distinct tissue-specific expression patterns and functional roles. UNC119B is notably expressed in the brain, retina, and immune cells, suggesting its importance in neural and immune system regulation.
Structurally, UNC119B contains an N-terminal SH3-binding domain and a central lipid-binding domain, enabling interactions with myristoylated or palmitoylated proteins. It acts as a chaperone for lipid-modified proteins, facilitating their proper trafficking and localization. For instance, UNC119B binds to Arf-like (ARL) GTPases, such as ARL2 and ARL3. which are essential for ciliogenesis and photoreceptor function. This interaction aids in nucleotide exchange, regulating GTPase activity and downstream signaling.
In neurons, UNC119B is implicated in synaptic vesicle cycling and neurotransmitter release by modulating the transport of lipid-anchored proteins like Gα subunits of heterotrimeric G-proteins. Dysregulation of UNC119B has been linked to neurological disorders, including intellectual disability and retinal degeneration. Additionally, its role in immune cells involves T-cell receptor signaling and lipid raft organization, influencing immune response efficacy.
Recombinant UNC119B protein is engineered for *in vitro* studies to dissect its molecular mechanisms, protein-protein interactions, and therapeutic potential. Produced via bacterial or mammalian expression systems, it retains functional domains and post-translational modifications critical for activity. Research utilizing recombinant UNC119B has advanced understanding of ciliary disorders, neuropathies, and immune dysfunctions, highlighting its value as a tool for biomedical exploration and drug discovery.
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