Mouse Thyroid-Peroxidase,TPO ELISA Kit

货号: IPD-E08353m

Price： ￥2500

48T 96T

数量：

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产品描述：小鼠甲状腺过氧化物酶(TPO)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TPO含量。TPO（血小板生成素）是刺激巨核细胞生长及分化的内源性细胞因子。它作用于血小板生成各阶段，促进巨核祖细胞增殖和分化，增加血小板生成。其研究机制聚焦于明确信号传导通路等，对治疗血小板减少症等血液疾病意义重大。试剂盒检测范围为2.5 ng/mL-40 ng/mL，该产品特别适用于甲状腺功能调控机制研究、自身免疫性甲状腺疾病模型建立、药物或环境因素对甲状腺代谢影响的评估等场景，为探索甲状腺相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
缩写：TPO
Uniprot No.：P35419
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：2.5 ng/mL-40 ng/mL
灵敏度：2.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-103
1:2	Average %	89
	Range %	82-97
1:4	Average %	95
	Range %	87-100
1:8	Average %	90
	Range %	83-99

回收率：

The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-102
EDTA plasma (n=4)	95	90-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2Average 400.2720.2630.268 200.5250.5300.528 100.8940.8740.884 51.3341.3771.356 2.52.5542.5282.541

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