| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADCADN; CXXC finger protein 9; CXXC9; DNA methyltransferase 1; DNA MTase; Dnmt1o; HSN1E; M.HsaI; MCMT; Met1; MommeD2 |
| Entrez GeneID | 1786 |
| WB Predicted band size | Calculated MW: 183 kDa; Observed MW: 183 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Dnmt1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于Dnmt1抗体的3篇参考文献及其摘要概括：
1. **"Targeted mutation of the DNA methyltransferase gene results in embryonic lethality"**
*作者：Li, E., Bestor, T.H., Jaenisch, R. (1992)*
摘要：该研究利用Dnmt1抗体验证基因敲除小鼠模型中Dnmt1蛋白的缺失，发现Dnmt1缺失导致胚胎致死，强调了其在维持DNA甲基化和胚胎发育中的关键作用。
2. **"DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development"**
*作者：Okano, M., Bell, D.W., Haber, D.A., Li, E. (1999)*
摘要：研究通过Dnmt1抗体进行免疫印迹分析，对比Dnmt1与Dnmt3家族的功能差异，揭示了Dnmt1在维持甲基化中的特异性，而Dnmt3负责新甲基化模式的建立。
3. **"Abnormal regulation of DNA methyltransferase expression during colorectal carcinogenesis"**
*作者：Saito, Y., Kanai, Y., Nakagawa, T., et al. (2003)*
摘要：该文献使用Dnmt1抗体检测结直肠癌组织中Dnmt1蛋白表达水平，发现其异常上调与肿瘤进展相关，为表观遗传失调在癌症中的作用提供了证据。
**Background of Dnmt1 Antibody**
DNA methyltransferase 1 (Dnmt1) is a crucial enzyme responsible for maintaining DNA methylation patterns during replication, ensuring epigenetic stability by copying methylation marks onto newly synthesized DNA strands. As a maintenance methyltransferase, Dnmt1 preferentially binds to hemimethylated DNA at replication forks, preserving gene silencing and genomic imprinting. Its role in regulating gene expression, X-chromosome inactivation, and chromatin structure underscores its importance in development, cellular differentiation, and disease pathogenesis, particularly cancer.
Dnmt1-specific antibodies are essential tools for studying its expression, localization, and functional interactions. These antibodies enable detection of Dnmt1 in techniques like Western blotting (WB), immunohistochemistry (IHC), immunoprecipitation (IP), and immunofluorescence (IF). Researchers rely on validated Dnmt1 antibodies to explore its dysregulation in tumors, neurological disorders, and aging-related processes. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect diverse epitopes, enhancing flexibility across experimental setups.
Quality control, including knockout cell line validation, ensures antibody reliability. Studies using Dnmt1 antibodies have revealed its overexpression in cancers, linking it to hypermethylation of tumor suppressor genes, and its role in synaptic plasticity and neurodegeneration. As therapeutic targeting of Dnmt1 gains traction in oncology, these antibodies remain pivotal for mechanistic and translational research.
×
