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产品描述：人硫嘌呤S-甲基转换酶(TPMT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TPMT含量。TPMT即硫嘌呤甲基转移酶，是一种参与硫嘌呤类药物代谢的酶。其基因具有多态性，不同基因型表达的酶活性不同。研究机制主要围绕其基因多态性与硫嘌呤类药物疗效及毒副作用的关联，以实现用药的个体化和精准化。试剂盒检测范围为1.56 mU/mL-100 mU/mL，适用于科研领域探究硫嘌呤类药物代谢机制、评估患者个体化用药方案或开展TPMT遗传多态性相关研究；支持科研人员高效开展药物代谢动力学、酶活性调控及疾病机制等基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HGNC:12014 ELISA Kit; S adenosyl L methionine thiopurine S methyltransferase ELISA Kit; Thiopurine methyltransferase ELISA Kit; Thiopurine S methyltransferase ELISA Kit; Thiopurine S-methyltransferase ELISA Kit; TPMT ELISA Kit; TPMT_HUMAN ELISA Kit
缩写：TPMT
Uniprot No.：P51580
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 mU/mL-100 mU/mL
灵敏度：0.39 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TPMT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 90-105 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 91-110 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human TPMT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 85-100 | ||||
| EDTA plasma (n=4) | 92 | 80-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected1002.2392.1522.1962.044501.8541.7601.8071.655251.3111.2941.3031.15112.50.8430.8390.8410.6896.250.5710.5340.5530.4013.120.3800.3590.3700.2181.560.2430.2390.2410.08900.1530.1510.152 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TPMT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled TPMT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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