| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4 |
| Entrez GeneID | 3981 |
| WB Predicted band size | Calculated MW: 104 kDa; Observed MW: 104 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human DNA Ligase IV |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与DNA Ligase IV抗体相关的文献摘要(基于公开信息整理，具体内容建议核对原文)：
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1. **"Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein"**
- **作者**: Grawunder, U. et al.
- **摘要**: 该研究首次报道DNA Ligase IV与XRCC4形成复合物，并揭示其参与非同源末端连接(NHEJ)修复。文中使用特异性抗体验证Ligase IV在哺乳动物细胞中的表达及与XRCC4的相互作用，为后续功能研究奠定基础。
2. **"A pathway of double-strand break rejoining dependent upon ATM, Artemis, and proteins locating to γ-H2AX foci"**
- **作者**: Riballo, E. et al.
- **摘要**: 通过免疫沉淀和Western blot分析，研究证实DNA Ligase IV在ATM激酶调控的DNA修复通路中不可或缺。抗体用于检测细胞辐射后Ligase IV的磷酸化状态及亚细胞定位变化。
3. **"The mechanism of human nonhomologous DNA end joining"**
- **作者**: Lieber, M.R. et al.
- **摘要**: 综述中总结了Ligase IV在NHEJ中的核心作用，并提到利用特异性抗体阻断其功能可导致修复缺陷，证明其在V(D)J重组和基因组稳定性中的必要性。
4. **"XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining"**
- **作者**: Ahnesorg, P. et al.
- **摘要**: 研究通过免疫共沉淀(Co-IP)和抗体介导的蛋白互作分析，揭示XLF蛋白与Ligase IV-XRCC4复合物的协同作用，抗体用于验证基因敲除细胞中Ligase IV的缺失表型。
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注：以上信息基于领域内经典文献概括，如需准确引用请查阅原文并核对实验细节。
DNA Ligase IV is a critical enzyme involved in the repair of DNA double-strand breaks (DSBs), primarily through the non-homologous end joining (NHEJ) pathway. It functions by catalyzing the final phosphodiester bond formation during DNA end ligation, a process essential for maintaining genomic stability. Ligase IV operates in a complex with XRCC4 and interacts with other repair factors like XLF (XRCC4-like factor) to facilitate efficient DSB repair. Its activity is crucial for V(D)J recombination during immune system development and class-switch recombination in B cells. Mutations in the *LIG4* gene are linked to severe disorders, including LIG4 syndrome, characterized by immunodeficiency, developmental defects, and radiation sensitivity.
Antibodies targeting DNA Ligase IV are widely used to study its expression, localization, and interactions in cellular contexts. These antibodies enable detection via techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Specific clones or polyclonal preparations are validated for reactivity in human, mouse, or rat models, often confirmed using knockout cell lines to ensure specificity. Researchers employ these tools to investigate DNA repair mechanisms, genomic instability in cancer, and the molecular basis of immune disorders. Commercial antibodies typically provide data on application-specific validation, supporting their utility in both basic research and clinical diagnostics.
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