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产品描述：人硫胺焦磷酸激酶1(TPK1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TPK1含量。人硫胺焦磷酸激酶1（TPK1）是一种催化硫胺素转化为焦磷酸硫胺素的酶，参与糖酵解和能量代谢。研究显示，TPK1基因突变可导致硫胺素代谢功能障碍综合征5（TMDS5），影响TPP生成，引发多种神经发育和运动障碍。通过基因检测和NGS技术，可确定TPK1基因变异情况，为疾病诊断和治疗提供依据。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于探究TPK1在神经系统疾病、代谢紊乱及硫胺素缺乏相关病理机制中的表达变化，为研究硫胺素代谢调控、评估营养干预效果或探索相关疾病的分子机制提供可靠的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：hTPK1 ELISA Kit; Placental protein 20 ELISA Kit; PP20 ELISA Kit; Thiamin pyrophosphokinase 1 ELISA Kit; Thiamine diphosphokinase ELISA Kit; Thiamine kinase ELISA Kit; Thiamine pyrophosphokinase 1 ELISA Kit; THMD5 ELISA Kit; TPK1 ELISA Kit; TPK1_HUMAN ELISA Kit
缩写：TPK1
Uniprot No.：Q9H3S4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TPK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human TPK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.3292.3682.3492.24310001.8761.8571.8671.7615001.3391.3681.3541.2482500.8430.8390.8410.7351250.5660.5990.5830.47762.50.4110.4230.4170.31131.250.2500.2590.2550.14900.1050.1070.106 | ||||||
货期：3-5 working days
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