首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠色氨酸5羟化酶2(TPH2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TPH2含量。TPH2即色氨酸羟化酶 2，是合成脑内血清素的限速酶。其功能与情绪、认知等多种神经活动紧密相关。研究聚焦于其在抑郁症等精神疾病发病机制中的作用，探索通过调节 TPH2 活性来干预血清素合成，为治疗此类疾病提供新思路。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于神经科学、精神疾病模型研究或药物干预实验中TPH2的动态监测，例如探究抑郁症、焦虑症等疾病模型中5-HT系统的调控机制，或评估神经活性药物对TPH2表达的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tph2 ELISA Kit; Ntph ELISA Kit; Tryptophan 5-hydroxylase 2 ELISA Kit; EC 1.14.16.4 ELISA Kit; Neuronal tryptophan hydroxylase ELISA Kit; Tryptophan 5-monooxygenase 2 ELISA Kit
缩写：TPH2
Uniprot No.：Q8CGU9
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TPH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 93-102 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 96-105 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 87-95 | ||||||
回收率：
| The recovery of rat TPH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 89-96 | |||||
| EDTA plasma (n=4) | 97 | 90-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.1232.2622.1932.092 10001.4951.5851.5401.439 5001.0121.0441.0280.927 2500.6640.6770.6710.570 1250.4420.4510.4470.346 62.50.2760.2890.2830.182 31.250.1910.1950.1930.092 00.1020.1000.101 | |||||||
货期：3-5 working days
×
