首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠色氨酸羟化酶(TPH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TPH1含量。TPH1（色氨酸羟化酶 1）是一种重要靶点。它主要参与血清素合成，在调节情绪、睡眠、食欲等生理过程中发挥关键作用。研究聚焦于其在神经精神疾病如抑郁症、焦虑症中的机制，旨在通过调控 TPH1 活性来开发新的治疗手段。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，用于探索5 - 羟色胺相关信号通路、构建情绪障碍动物模型、分析代谢调控机制或评估药物对神经递质合成的影响等科研场景；助力科研人员深入解析其在生理及病理状态下的生物学功能本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tph1 ELISA Kit; Tph ELISA Kit; Tryptophan 5-hydroxylase 1 ELISA Kit; EC 1.14.16.4 ELISA Kit; Tryptophan 5-monooxygenase 1 ELISA Kit
缩写：TPH1
Uniprot No.：P09810
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：37.5 pg/mL-2400 pg/mL
灵敏度：9.4 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TPH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 94-100 | ||||||
| 1:2 | Average % | 88 | |||||
| Range % | 83-96 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-97 | ||||||
| 1:8 | Average % | 111 | |||||
| Range % | 106-115 | ||||||
回收率：
| The recovery of rat TPH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 82-96 | |||||
| EDTA plasma (n=4) | 105 | 103-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected24001.8211.8311.8261.69212001.4881.4521.4701.3366001.1511.2181.1851.0513000.8540.8970.8760.7421500.5110.5400.5260.392750.3100.3110.3110.17737.50.2660.2540.2600.12600.1380.1290.134 | |||||||
货期：3-5 working days
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