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Rabbit Monoclonal DHX8 Antibody

  • 中文名： DHX8抗体
  • 别    名： DDX8; Dhr2; HRH1; PRP22; PRPF22
货号: IPDX21315
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDDX8; Dhr2; HRH1; PRP22; PRPF22
Entrez GeneID1659
WB Predicted band sizeCalculated MW: 139 kDa; Observed MW: 139 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human DHX8
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于DHX8抗体的3篇参考文献示例(注：文献信息为假设性示例，实际文献需通过学术数据库验证)：

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1. **文献名称**: "DHX8 regulates pre-mRNA splicing through interaction with the spliceosome complex"

**作者**: Tanaka H, et al.

**摘要**: 本研究利用DHX8特异性抗体进行免疫共沉淀实验，揭示了DHX8作为RNA解旋酶在剪接体拆卸阶段的关键作用。实验表明，DHX8通过与SF3B1等剪接相关蛋白相互作用，调控pre-mRNA的剪接效率。

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2. **文献名称**: "DHX8 depletion disrupts DNA damage response and promotes genomic instability"

**作者**: Zhou Y, et al.

**摘要**: 通过Western blot和免疫荧光技术结合DHX8抗体，研究发现DHX8缺失导致DNA损伤修复信号通路(如ATM/ATR)异常，并引发染色体断裂，提示DHX8在维持基因组稳定性中的新功能。

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3. **文献名称**: "DHX8 interacts with viral RNA to restrict influenza A virus replication"

**作者**: Liu X, et al.

**摘要**: 本文使用DHX8抗体进行蛋白质-RNA结合实验，证明DHX8通过识别病毒RNA并招募抗病毒因子抑制流感病毒复制，为宿主先天免疫机制提供了新见解。

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如需具体文献，建议通过PubMed或Google Scholar检索关键词“DHX8 antibody”、“DHX8 function”或结合研究领域(如“RNA splicing”、“viral infection”)筛选近期论文。实际研究中，抗体相关描述通常出现在方法学部分(如“Antibodies used: anti-DHX8. Sigma, #AB1234”)或功能验证实验中。

背景信息

The DHX8 antibody is a crucial tool for studying DHX8 (DEAH-box helicase 8), a member of the DEAH-box RNA helicase family involved in pre-mRNA splicing and RNA metabolism. DHX8 plays a pivotal role in the later stages of spliceosome assembly, specifically in the disassembly of the intron-lariat spliceosome (ILS) complex, ensuring proper mRNA maturation and export to the cytoplasm. Its functions are linked to genome stability, cell cycle regulation, and antiviral responses. Dysregulation of DHX8 has been implicated in cancers, neurodevelopmental disorders, and viral infections. Researchers utilize DHX8 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions with spliceosome components (e.g., PRPF8. SNW1) or viral proteins. Commercial DHX8 antibodies are typically raised against specific epitopes (e.g., human DHX8 amino acids 500-800) and validated for specificity across species (human, mouse, rat). Studies employing these antibodies have revealed DHX8’s role in alternative splicing in diseases and its potential as a therapeutic target. Proper controls (e.g., siRNA knockdown) are essential to confirm antibody specificity in experimental settings.

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