| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha |
| Entrez GeneID | 54487 |
| WB Predicted band size | Calculated MW: 86 kDa; Observed MW: 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human DGCR8 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DGCR8抗体的3篇参考文献及其摘要内容：
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1. **"The Drosha-DGCR8 complex in primary microRNA processing"**
*作者：Han, J., Lee, Y., Yeom, K.H., et al.*
**摘要**：该研究通过免疫共沉淀和Western blot技术，证实DGCR8与Drosha形成复合体，共同参与pri-miRNA的切割加工。DGCR8抗体的应用揭示了其在RNA结合及复合体稳定性中的关键作用。
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2. **"Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex"**
*作者：Landthaler, M., Yalcin, A., Tuschl, T.*
**摘要**：文章利用DGCR8抗体进行RNA干扰实验，发现DGCR8通过结合pri-miRNA的茎环结构引导Drosha精确定位切割位点，阐明了其在microRNA成熟中的分子机制。
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3. **"DGCR8-dependent stress-induced miRNA biogenesis in human cells"**
*作者：Shibata, S., Sasaki, M., Mita, T., et al.*
**摘要**：研究使用DGCR8抗体验证其在细胞应激条件下的表达变化，发现DGCR8通过调节特定miRNA生成参与细胞应激反应通路，提示其潜在的疾病治疗靶点价值。
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这些文献展示了DGCR8抗体在解析microRNA加工机制、结构功能及疾病关联中的关键应用。如需更多方向(如神经疾病或癌症研究)，可进一步补充。
The DGCR8 antibody is a crucial tool for studying the DGCR8 (DiGeorge Syndrome Critical Region Gene 8) protein, a key component of the microprocessor complex involved in microRNA (miRNA) biogenesis. DGCR8 partners with the RNase III enzyme Drosha to cleave primary miRNA transcripts (pri-miRNAs) into precursor miRNAs (pre-miRNAs) in the nucleus. This process is essential for post-transcriptional gene regulation, impacting cellular processes like development, differentiation, and disease pathogenesis. DGCR8 is located within the 22q11.2 chromosomal region, deletions of which are linked to DiGeorge syndrome and an increased risk of neuropsychiatric disorders such as schizophrenia.
Researchers use DGCR8 antibodies to investigate its expression, localization, and interactions in various biological contexts. These antibodies are widely applied in techniques like Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP) to assess DGCR8's role in miRNA processing and its dysregulation in diseases. Specific DGCR8 antibodies are validated for species cross-reactivity (e.g., human, mouse, rat) and tested for specificity to avoid cross-reactivity with related proteins. Their utility extends to both basic research—exploring miRNA biogenesis mechanisms—and clinical studies, such as examining DGCR8's involvement in cancer or neurodevelopmental disorders. Commercial DGCR8 antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal variants available to suit different experimental needs.
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