首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠P53(P53)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TP53含量。TP53是重要的抑癌基因，也被称“基因组卫士”。其能监控细胞基因组完整性，通过调控下游基因表达来修复受损DNA或诱导细胞凋亡。当发生突变时，会失去正常功能，与多种肿瘤发生发展密切相关，是肿瘤研究的热门靶点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于体外科研场景中P53蛋白的定量分析，例如肿瘤动物模型研究、基因表达调控实验或细胞应激状态下蛋白动态变化的监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TP53
Uniprot No.：P10361
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat p53/TP53 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 95-108 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of rat p53/TP53 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-93 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.4792.6152.5472.4054002.0091.9741.9921.8502001.3561.3301.3431.2011000.8740.9290.9020.760500.5320.5710.5520.410250.4010.4250.4130.27112.50.2970.2870.2920.15000.1400.1430.142 | ||||||
货期：3-5 working days
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