首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人P53(P53)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、脑脊液（CSF）、组织匀浆、细胞裂解物样本中的TP53含量。TP53是重要的抑癌基因。其它在细胞周期调控、DNA 损伤修复等过程中起着关键作用。研究机制上，正常时可抑制细胞恶性增殖，突变后则可能失去抑癌功能，还与肿瘤发生、发展及预后密切相关，是肿瘤研究的重要靶点。试剂盒检测范围为9.38 pg/mL-600 pg/mL，该产品适用于基础科研领域，可用于探究肿瘤发生机制、药物干预对P53表达的影响、细胞应激反应研究等方向，例如通过检测不同处理条件下细胞裂解液或动物模型组织样本中的P53动态变化，分析其在特定信号通路中的作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Antigen NY-CO-13 ELISA Kit; BCC7 ELISA Kit; Cellular tumor antigen p53 ELISA Kit; FLJ92943 ELISA Kit; LFS1 ELISA Kit; Mutant tumor protein 53 ELISA Kit; p53 ELISA Kit; p53 tumor suppressor ELISA Kit; P53_HUMAN ELISA Kit; Phosphoprotein p53 ELISA Kit; Tp53 ELISA Kit; Transformation related protein 53 ELISA Kit; TRP53 ELISA Kit; tumor antigen p55 ELISA Kit; Tumor protein 53 ELISA Kit; Tumor protein p53 ELISA Kit; Tumor suppressor p53 ELISA Kit
缩写：TP53
Uniprot No.：P04637
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
检测范围：9.38 pg/mL-600 pg/mL
灵敏度：2.34 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human p53/TP53 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 80-110 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human p53/TP53 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 89-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected6002.6232.6762.6502.5203002.2132.2862.2502.1201501.6781.6981.6881.558751.0591.0891.0740.94437.50.6480.6890.6690.53918.70.4410.4800.4610.3319.380.2980.3000.2990.16900.1330.1270.130 | ||||||
货期：3-5 working days
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