首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠心肌肌钙蛋白T(cTn-T)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的cTn-T含量。cTn-T即心肌肌钙蛋白T，是心肌损伤的特异性标志物。其背景在于临床上用于诊断心肌梗死等疾病。研究机制方面，心肌受损时，cTn-T释放入血，通过检测血液中其含量变化，可反映心肌损伤程度、范围及评估预后等。试剂盒检测范围为12.5 pg/mL-800 pg/mL，可广泛应用于心脏疾病动物模型研究，如心肌缺血再灌注损伤、药物心脏毒性评估、心肌炎或心力衰竭机制探索等科研领域，为体外定量分析心肌损伤提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnnt2 ELISA Kit; Troponin T ELISA Kit; cardiac muscle ELISA Kit; TnTc ELISA Kit; Cardiac muscle troponin T ELISA Kit; cTnT ELISA Kit
缩写：cTn-T
Uniprot No.：P50753
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-T in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-97 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 81-94 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-102 | |||||
回收率：
| The recovery of rat cTn-T spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-105 | ||||
| EDTA plasma (n=4) | 95 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.1872.1022.1451.9864001.7311.6411.6861.5272001.2541.2041.2291.0701000.9260.8790.9030.744500.5930.5540.5740.415250.3020.2790.2910.13212.50.2410.2270.2340.07500.1650.1530.159 | ||||||
货期：3-5 working days
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