| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GUA; GURDB; RH-II/GU; RH-II/GuA |
| Entrez GeneID | 9188 |
| WB Predicted band size | Calculated MW: 87 kDa; Observed MW: 87 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human DDX21 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DDX21抗体的3篇参考文献及其摘要概括：
1. **"DEAD-box helicase DDX21 coordinates transcriptional and ribosomal RNA processing"**
- **作者**: Calo E. et al. (2015. *Cell Reports*)
- **摘要**: 该研究揭示了DDX21在调控核糖体RNA(rRNA)合成和转录延伸中的双重作用。通过免疫沉淀(IP)和染色质免疫沉淀(ChIP)实验，结合DDX21抗体，发现其与RNA聚合酶I/II相互作用，并参与核仁应激反应。研究还表明，DDX21缺失导致rRNA加工异常和转录暂停。
2. **"DDX21 mediates co-transcriptional RNA m6A modification to promote transcription termination and genome stability"**
- **作者**: Zhang Y. et al. (2021. *Molecular Cell*)
- **摘要**: 本文利用DDX21抗体进行免疫荧光和RNA免疫沉淀测序(RIP-seq)，发现DDX21通过结合m6A修饰酶复合物，调控RNA的共转录修饰。研究表明，DDX21缺失导致转录终止缺陷和DNA损伤，强调了其在维持基因组稳定性中的关键作用。
3. **"The DEAD-box protein DDX21 is a viral restriction factor that activates RNase L"**
- **作者**: Li Y. et al. (2020. *Cell Host & Microbe*)
- **摘要**: 该研究通过Western blot和免疫共沉淀(Co-IP)实验，结合DDX21抗体，揭示了其在抗病毒先天免疫中的新功能。DDX21被证实可识别病毒RNA，并通过激活RNase L通路抑制病毒复制(如甲型流感病毒)。研究还发现，病毒蛋白通过降解DDX21逃逸宿主防御。
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这些文献展示了DDX21抗体在多种实验中的应用，包括定位分析、蛋白互作研究及功能机制探索，涉及RNA代谢、抗病毒免疫和基因组稳定性等方向。
DDX21 (DExD-box helicase 21) is an RNA helicase belonging to the DEAD-box protein family, characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. It plays multifaceted roles in RNA metabolism, including ribosome biogenesis, transcriptional regulation, and innate immune response. DDX21 is involved in resolving RNA secondary structures, facilitating ribosomal RNA (rRNA) processing, and modulating the transcription of genes linked to cell proliferation and antiviral defense. Dysregulation of DDX21 has been implicated in cancers, neurodegenerative disorders, and viral infections, highlighting its biological and clinical relevance.
DDX21 antibodies are essential tools for studying its expression, subcellular localization, and molecular interactions. These antibodies enable detection of DDX21 in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Given DDX21's dual nuclear and nucleolar distribution, specific antibodies help elucidate its dynamic shuttling during stress or infection. Researchers also use DDX21 antibodies to explore its role in viral replication cycles, as some pathogens exploit DDX21 to enhance their propagation. Commercial DDX21 antibodies are typically raised against conserved epitopes, with validation in knockdown/knockout models to ensure specificity. Variations in antibody clones (e.g., mouse or rabbit monoclonal) may affect performance across applications, necessitating protocol optimization. Reliable DDX21 antibodies are critical for advancing studies in cancer biology, virology, and developmental disorders.
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