首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人心肌肌钙蛋白T(cTn-T)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TNNT1含量。TNNT1是重要的靶点。它是心肌肌钙蛋白亚基，在心肌收缩调节中起关键作用。对它的研究机制主要聚焦于其与心肌疾病的关联，探索其在疾病发生发展中的作用，有望为相关心肌疾病的诊断、治疗及药物研发提供方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品采用预包被特异性抗体的96孔板结构，配套标准品、检测抗体及显色试剂，操作流程标准化，支持科研领域中心血管疾病机制研究、药物心脏毒性评估以及心肌损伤动物模型分析等应用场景，为探索心肌病理生理过程提供高灵敏度的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ANM ELISA Kit; MGC104241 ELISA Kit; NEM5 ELISA Kit; Skeletal ELISA Kit; Slow ELISA Kit; slow skeletal muscle ELISA Kit; Slow skeletal muscle troponin T ELISA Kit; sTnT ELISA Kit; Tnnt1 ELISA Kit; TNNT1_HUMAN ELISA Kit; TNT ELISA Kit; TnTs ELISA Kit; Troponin T ELISA Kit; Troponin T slow skeletal muscle ELISA Kit; Troponin T type 1 (skeletal slow) ELISA Kit; troponin T1 skeletal slow ELISA Kit; Troponin T1, slow skeletal type ELISA Kit
缩写：TNNT1
Uniprot No.：P13805
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human cTn-T in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-107 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human cTn-T spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-104 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2882.3912.3402.19451.4471.5081.4781.3322.50.8240.8380.8310.6851.250.5250.5470.5360.3900.6250.3160.3290.3230.1770.3120.2640.2730.2690.1230.1560.2250.2340.2300.08400.1450.1460.146 | ||||||
货期：3-5 working days
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