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Rabbit Monoclonal DBF4 Antibody

  • 中文名： DBF4抗体
  • 别    名： DBF4; ASK; DBF4A; ZDBF1; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1
货号: IPDX21300
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDBF4; ASK; DBF4A; ZDBF1; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1
Entrez GeneID10926
WB Predicted band sizeCalculated MW: 77 kDa; Observed MW: 77 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human DBF4
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于DBF4抗体的3-4条参考文献示例(注：文献为虚构示例，实际引用需根据真实研究调整)：

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1. **文献名称**: *"Regulation of DNA replication initiation by DBF4 phosphorylation in yeast"*

**作者**: Tanaka S, Diffley JFX

**摘要**: 本研究通过特异性抗体验证了DBF4蛋白在酿酒酵母细胞周期中的表达模式，发现其磷酸化状态调控CDC7激酶活性，进而影响DNA复制的起始时机。

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2. **文献名称**: *"Human DBF4 as a biomarker for replication stress in cancer cells"*

**作者**: Nishitani H, Lygerou Z

**摘要**: 利用DBF4抗体进行免疫印迹分析，揭示了在癌细胞中DBF4表达水平与复制压力响应之间的相关性，表明其可作为治疗靶点的潜力。

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3. **文献名称**: *"Dbf4-dependent kinase recruitment to chromatin under replication stress"*

**作者**: Urban RA, et al.

**摘要**: 研究通过ChIP实验结合DBF4抗体，证实DBF4-CDC7复合物在复制压力下被招募到染色质损伤位点，促进复制叉的稳定性修复。

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4. **文献名称**: *"Cell cycle-dependent degradation of DBF4 ensures timely mitotic entry"*

**作者**: Kanemaki MT, Walter JC

**摘要**: 使用DBF4抗体追踪蛋白稳定性，发现其通过泛素化途径在G2/M期降解，防止DNA复制重启，确保有丝分裂的正常进行。

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**注意**：以上文献为示例，实际研究中请通过数据库(如PubMed、Google Scholar)检索真实发表的论文，并核对作者、标题及摘要内容。

背景信息

The DBF4 antibody is a research tool designed to detect DBF4 (Dbf4-dependent kinase), a regulatory subunit of the Cdc7-Dbf4 kinase complex essential for initiating DNA replication during the cell cycle. DBF4 interacts with the serine/threonine kinase CDC7 to form an active complex that phosphorylates minichromosome maintenance (MCM) proteins, promoting the unwinding of DNA and firing of replication origins during the S phase. This kinase complex is tightly regulated, with DBF4 expression peaking at the G1/S transition and declining after replication. Antibodies targeting DBF4 are widely used in studies exploring cell cycle regulation, DNA replication mechanisms, and genomic stability. They enable detection of DBF4 protein levels via techniques like Western blotting, immunofluorescence, or immunoprecipitation, often in models ranging from yeast to human cells. Dysregulation of the Cdc7-DBF4 axis is linked to cancer progression, as many tumors overexpress these proteins to sustain uncontrolled proliferation. Consequently, DBF4 antibodies also serve as biomarkers in oncology research, particularly in studies evaluating replication stress or therapeutic inhibitors targeting DNA replication kinases. Commercial DBF4 antibodies are typically validated for specificity across species, including human, mouse, and rat samples.

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