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产品描述：兔心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNNI3含量。TNNI3是重要的心肌肌钙蛋白亚基，在心肌收缩和舒张调节中发挥关键作用。其异常与多种心脏疾病密切相关，如心肌梗死、心肌病等。科研人员通过研究其表达、结构及功能机制，探索基于TNNI3的诊断标志物和治疗靶点。试剂盒检测范围为47 pg/mL-3000 pg/mL，该产品适用于基础科研中与心肌损伤相关的动物模型研究，如心肌缺血再灌注损伤、药物心脏毒性评估或心血管疾病机制探讨等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
缩写：TNNI3
Uniprot No.：P02646
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of rabbit cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 83-94 | ||||
| EDTA plasma (n=4) | 89 | 85-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.7542.5592.6572.56415002.1152.0202.0681.9757501.2481.2271.2381.1453750.6580.6770.6680.575187.50.4030.4120.4080.315940.2640.2780.2710.178470.1510.1470.1490.05600.0930.0920.093 | ||||||
货期：3-5 working days
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