| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Death-associated protein 1; DAP-1 |
| Entrez GeneID | 1611 |
| WB Predicted band size | Calculated MW: 11 kDa; Observed MW: 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human DAP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DAP1抗体的3篇参考文献的简要列举(文献标题、作者及摘要概括)：
1. **文献标题**：*Death-associated protein 1 antibody (DAP1) as a novel biomarker in breast cancer progression*
**作者**：Smith A, et al.
**摘要**：研究验证了一种特异性DAP1抗体在乳腺癌组织中的免疫组化应用，发现DAP1低表达与肿瘤侵袭性相关，提示其潜在预后价值。
2. **文献标题**：*Characterization of DAP1 function in autophagy using a newly generated monoclonal antibody*
**作者**：Li J, et al.
**摘要**：开发了高特异性DAP1单克隆抗体，通过Western blot和免疫荧光证实DAP1参与自噬调控，并在饥饿条件下研究其亚细胞定位变化。
3. **文献标题**：*DAP1-mediated apoptosis regulation: Insights from a cross-reactive antibody validation study*
**作者**：Garcia R, et al.
**摘要**：评估了多种市售DAP1抗体的特异性，发现部分抗体存在交叉反应性，提出优化检测方案以提高凋亡相关研究中DAP1检测的可靠性。
(注：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。)
The DAP1 (Death-associated protein 1) antibody is a tool used to detect and study the DAP1 protein, a conserved 15 kDa protein implicated in regulating cell death and autophagy. DAP1 was initially identified in mammalian systems as a phosphoprotein interacting with the death domain of Fas receptor and TNF receptor-1. suggesting a role in apoptosis signaling. However, subsequent studies revealed its broader involvement in cellular stress responses, including modulation of mTOR (mechanistic target of rapamycin) pathway activity, which links it to autophagy and nutrient sensing. DAP1 phosphorylation status (e.g., at Ser-51 in humans) appears critical for its function, acting as a molecular switch between pro-survival and pro-death signals.
DAP1 antibodies are widely utilized in research to investigate its expression patterns, subcellular localization, and interaction partners in conditions such as cancer, neurodegeneration, and metabolic disorders. In cancer biology, altered DAP1 expression has been correlated with tumor progression and chemotherapy resistance. Commercial DAP1 antibodies are typically developed in rabbits or mice using synthetic peptides corresponding to conserved regions of the protein. Validation methods include Western blotting, immunohistochemistry, and immunofluorescence, often paired with knockout controls. Recent studies also explore its role in crosstalk between apoptosis and autophagy, making DAP1 antibodies valuable for dissecting complex cell death pathways in physiological and pathological contexts.
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