| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; K 18; K18; K1C18_HUMAN; Keratin 18; Keratin D; keratin; type I cytoskeletal 18; Keratin-18; KRT18. |
| Entrez GeneID | 3875 |
| WB Predicted band size | Calculated MW: 48 kDa; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human Cytokeratin 18 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Cytokeratin 18抗体的3篇参考文献的简要概括：
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1. **文献名称**：*Caspase-mediated cleavage of cytokeratin 18 is associated with apoptosis in epithelial cells*
**作者**：Schutte B, et al.
**摘要**：该研究通过Cytokeratin 18(CK18)抗体(如M30抗体)检测caspase切割的CK18片段(CK18-Asp396)，揭示了其在细胞凋亡中的特异性标记作用，为上皮细胞凋亡的体外和体内检测提供了重要工具。
2. **文献名称**：*Cytokeratin 18 as a marker of tumor progression in hepatocellular carcinoma*
**作者**：Ueno T, et al.
**摘要**：研究利用CK18抗体分析肝细胞癌(HCC)患者的组织及血清样本，发现CK18表达水平与肿瘤分化程度和患者预后相关，提示其作为HCC诊断和预后评估的潜在生物标志物。
3. **文献名称**：*Immunohistochemical localization of cytokeratin 18 in epithelial tumors*
**作者**：Moll R, et al.
**摘要**：通过免疫组化技术系统研究了CK18抗体在不同上皮源性肿瘤(如腺癌、鳞癌)中的表达模式，证实CK18在腺上皮肿瘤中高表达，可用于辅助鉴别肿瘤组织来源。
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如需更具体的文献年份或期刊信息，可结合PubMed或Google Scholar进一步查询。
Cytokeratin 18 (CK18) is a type I intermediate filament protein predominantly expressed in single-layer epithelial tissues and their derived carcinomas. As a member of the cytokeratin family, CK18 pairs with type II keratins (e.g., CK8) to form cytoskeletal networks, providing mechanical stability and regulating cellular processes like apoptosis and stress response. CK18 antibodies are essential tools in research and diagnostics, targeting specific epitopes of the protein for detecting epithelial differentiation in tissues or cells. Commonly used clones include DC10 and KS20.20. which recognize different epitopes and are employed in immunohistochemistry (IHC) and Western blotting to identify carcinomas of epithelial origin (e.g., adenocarcinomas) and distinguish them from non-epithelial tumors.
In cancer research, CK18 antibodies aid in studying tumor progression, metastasis, and drug resistance. Elevated CK18 levels in serum, often detected via ELISA, are associated with epithelial cell death in conditions like liver injury or chemotherapy response. Additionally, caspase-cleaved CK18 fragments (detected by M30 antibody) serve as apoptosis biomarkers, while intact CK18 (M65 antibody) indicates necrotic cell death. These applications make CK18 antibodies valuable in oncology, hepatology, and toxicology. However, interpretation requires caution due to variable expression across cancer subtypes and potential cross-reactivity with other keratins. Proper validation and context-specific controls are critical for accurate analysis.
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