首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肿瘤坏死因子β(TNF-β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNF-β含量。TNF-β，又称淋巴毒素-α，是肿瘤坏死因子超家族成员，由淋巴细胞产生。其激活NF-κB信号通路，诱导癌细胞增殖、侵袭，并介导多种炎症、免疫和抗病毒反应。研究其机制有助于开发针对肿瘤、炎症等疾病的药物。试剂盒检测范围为7.8 pg/mL-500 pg/mL，尤其适用于动物模型研究中TNF-β的动态监测，例如炎症性疾病建模、免疫调节实验或抗肿瘤药物作用评估等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
缩写：TNF-β
Uniprot No.：Q06332
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 83-94 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 91-102 | ||||||
回收率：
| The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 99108 | |||||
| EDTA plasma (n=4) | 88 | 84-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.6322.7652.6992.6142502.2122.3022.2572.1721251.5451.5771.5611.47662.50.7650.7710.7680.68331.20.4110.4230.4170.33215.60.2570.2640.2610.1767.80.1760.1850.1810.09600.0840.0850.085 | |||||||
货期：3-5 working days
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