| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UGP2 |
| Uniprot No | Q16851 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-508aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSRFVQDLSKAMSQDGASQFQEVIRQE LELSVKKELEKILTTASSHEFEHTKKDLDGFRKLFHRFLQEKGPSVDWGK IQRPPEDSIQPYEKIKARGLPDNISSVLNKLVVVKLNGGLGTSMGCKGPK SLIGVRNENTFLDLTVQQIEHLNKTYNTDVPLVLMNSFNTDEDTKKILQK YNHCRVKIYTFNQSRYPRINKESLLPVAKDVSYSGENTEAWYPPGHGDIY ASFYNSGLLDTFIGEGKEYIFVSNIDNLGATVDLYILNHLMNPPNGKRCE FVMEVTNKTRADVKGGTLTQYEGKLRLVEIAQVPKAHVDEFKSVSKFKIF NTNNLWISLAAVKRLQEQNAIDMEIIVNAKTLDGGLNVIQLETAVGAAIK SFENSLGINVPRSRFLPVKTTSDLLLVMSNLYSLNAGSLTMSEKREFPTV PLVKLGSSFTKVQDYLRRFESIPDMLELDHLTVSGDVTFGKNVSLKGTVI IIANHGDRIDIPPGAVLENKIVSGNLRILDH |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UGP2重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际文献需在学术数据库中检索确认)：
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1. **标题**: *Heterologous Expression and Characterization of Recombinant UGP2 from Arabidopsis thaliana in Escherichia coli*
**作者**: Zhang L, Wang Y, et al.
**摘要**: 本研究报道了拟南芥UGP2基因在大肠杆菌中的重组表达及酶学性质分析。通过优化表达条件获得可溶性重组蛋白，并纯化后测定其催化UDP-葡萄糖合成的动力学参数，揭示了其底物特异性及在植物糖代谢中的潜在作用。
2. **标题**: *Structural Insights into Human UGP2: Crystallographic Analysis of the Recombinant Enzyme*
**作者**: Smith J, Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了人源UGP2重组蛋白的3D结构，揭示了其活性位点的关键氨基酸残基及与底物结合的分子机制。研究为开发针对UGP2的抑制剂提供了结构基础，可能应用于代谢性疾病治疗。
3. **标题**: *Functional Role of Recombinant UGP2 in Hepatic Glycogen Synthesis Using a Knockout Cell Model*
**作者**: Chen H, Li M, et al.
**摘要**: 利用CRISPR技术构建UGP2敲除的肝细胞系，通过外源表达重组UGP2蛋白，证实其恢复UDP-葡萄糖生成及糖原合成的能力，表明UGP2在肝脏能量稳态中的核心作用。
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如需获取真实文献，建议访问 **PubMed** (https://pubmed.ncbi.nlm.nih.gov) 或 **Google Scholar**，以关键词“UGP2 recombinant protein”“UGP2 expression and purification”检索。
**Background of UGP2 Recombinant Protein**
UGP2 (UDP-glucose pyrophosphorylase 2) is a key enzyme in carbohydrate metabolism, catalyzing the reversible conversion of glucose-1-phosphate and UTP to UDP-glucose, a critical precursor for glycogen synthesis, cell wall biosynthesis, and protein glycosylation. Encoded by the *UGP2* gene in humans, this enzyme is ubiquitously expressed, with high activity in tissues like liver, muscle, and brain, where glucose homeostasis and energy storage are vital. Unlike its isoform UGP1. which is found in plants and some bacteria, UGP2 is the dominant form in mammals and plays a non-redundant role in maintaining UDP-glucose pools essential for cellular functions.
Recombinant UGP2 protein is produced using biotechnological systems, such as *E. coli* or mammalian cell cultures, to express the purified enzyme for research and industrial applications. Its recombinant form retains enzymatic activity, enabling studies on glycogen metabolism disorders, such as glycogen storage diseases, and its regulatory mechanisms under conditions like diabetes or cancer. Structural analyses reveal UGP2’s conserved catalytic domains, which are targets for pharmacological modulation.
Beyond basic research, UGP2 recombinant protein is utilized in biomanufacturing to synthesize UDP-glucose-dependent glycoconjugates for therapeutic or diagnostic purposes. Recent studies also highlight its potential role in cancer progression, as elevated UGP2 expression correlates with tumor aggressiveness and chemoresistance, making it a candidate biomarker or therapeutic target. Overall, UGP2 recombinant protein serves as a vital tool for unraveling metabolic pathways and developing interventions for metabolic and neoplastic diseases.
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