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产品描述：鸡肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNF-α含量。TNF-α（肿瘤坏死因子-α）是一种多功能促炎细胞因子，主要在免疫应答、炎症反应和细胞凋亡中发挥作用。其研究机制涉及与受体TNFR1结合，激活多种信号通路，如线粒体途径、Bcl-2家族蛋白、活性氧等，导致细胞凋亡和炎症反应。TNF-α已成为治疗自身免疫疾病的关键靶点。试剂盒检测范围为0.27 pg/mL-200 pg/mL，适用于科研领域研究禽类传染性疾病（如禽流感、新城疫）的炎症反应机制，评估疫苗或抗炎药物对免疫系统的调控效果，以及构建家禽疾病模型时监测炎症因子动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
缩写：TNF-α
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：0.27 pg/mL-200 pg/mL
灵敏度：0.27 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 93-108 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.1730.1700.172 66.70.2590.2510.255 22.20.3470.3670.357 7.410.4520.4880.470 2.470.6110.6330.622 0.820.8090.8550.832 0.271.0331.0181.026 01.7481.7071.728 | ||||||
货期：3-5 working days
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