首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFsR2含量。TNFsR2 是重要靶点。TNF 家族参与多种生理病理过程，TNFsR2 作为其受体之一，在免疫调节、细胞存活与凋亡等方面发挥作用。研究聚焦其与配体结合机制，以及在疾病如炎症、肿瘤中的信号传导通路，旨在为相关疾病治疗提供新思路。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研实验中动态监测动物模型（如关节炎、脓毒症等）的炎症进程、评估药物干预对受体表达水平的影响，或探究TNF信号通路相关疾病的分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnfrsf1b ELISA Kit; Tnfr2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; TNF-R2 ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; TNF-RII ELISA Kit; TNFR-II ELISA Kit; p75 ELISA Kit; p80 TNF-alpha receptor ELISA Kit; CD antigen CD120b ELISA Kit
缩写：TNFsR-
Uniprot No.：Q80WY6
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 87-94 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 85-95 | ||||||
回收率：
| The recovery of rat TNFsR-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 88-96 | |||||
| EDTA plasma (n=4) | 98 | 95-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.8272.8992.8632.732202.2222.3232.2732.142101.3731.4121.3931.26250.8160.8660.8410.7102.50.5310.5450.5380.4071.250.3150.3230.3190.1880.6250.2260.2410.2340.10300.1260.1350.131 | |||||||
货期：3-5 working days
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