首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFsR1含量。TNFR1（Tumor Necrosis Factor Receptor 1），又称TNF-R55或CD120a，是一种与肿瘤坏死因子α（TNF-α）相互作用的受体蛋白，在多种生理和病理过程中发挥关键作用。其通过与TNF-α结合，触发信号传导，参与细胞凋亡、炎症反应和免疫调节等过程。研究显示，TNFR1在自身免疫疾病、炎症性疾病和癌症治疗中具有潜在应用价值。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，炎症机制研究、免疫相关疾病动物模型评估、药物干预效果分析、TNF/TNFR 信号通路调控、病理模型建立及生物标志物筛选等科研场景，适合需要多类型样本兼容的实验室使用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnfrsf1a; Tnfr-1; Tnfr1; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60; CD antigen CD120a
缩写：TNFsR-
Uniprot No.：P22934
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 100-110 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of rat TNFsR-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 82-93 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.1072.2212.1641.9875001.5621.4991.5311.3542500.9521.0030.9780.8011250.5730.5580.5660.38962.50.3950.4120.4040.22731.20.2840.2750.2800.10315.60.2290.2340.2320.05500.1810.1730.177 | |||||||
货期：3-5 working days
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