| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCNT; CYCT1; HIVE1 |
| Entrez GeneID | 904 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Cyclin T1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Cyclin T1抗体的3篇参考文献及其摘要内容：
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1. **文献名称**: *Cyclin T1: A Key Partner for HIV-1 Tat and CDK9 in Transcriptional Elongation*
**作者**: Wei P, Garber ME, et al.
**摘要**: 该研究揭示了Cyclin T1与CDK9形成P-TEFb复合物，通过与HIV Tat蛋白结合促进病毒转录延伸。通过Cyclin T1抗体的免疫共沉淀实验，证实了其在HIV复制中的关键作用。
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2. **文献名称**: *Regulation of Cyclin T1 Expression and Function by Phosphorylation*
**作者**: Nekhai S, Shukla RR, et al.
**摘要**: 研究探讨了Cyclin T1的磷酸化修饰对其功能的影响。利用特异性Cyclin T1抗体进行Western blot分析，发现其磷酸化状态调控P-TEFb复合物的亚细胞定位及转录活性。
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3. **文献名称**: *Cyclin T1 Overexpression in Hematologic Malignancies: Diagnostic Implications*
**作者**: Bacon CW, D’Orso I.
**摘要**: 通过免疫组化(IHC)和流式细胞术结合Cyclin T1抗体，研究发现Cyclin T1在急性白血病等血液肿瘤中异常高表达，提示其可作为潜在诊断标志物及治疗靶点。
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4. **文献名称**: *Structural Basis of Cyclin T1 Recruitment to the AFF4 Scaffold in the Super Elongation Complex*
**作者**: Garber ME, Jones KA.
**摘要**: 该研究通过Cyclin T1抗体的ChIP-seq和共聚焦显微技术，阐明了其与AFF4等蛋白形成超延伸复合物(SEC)的结构机制，推动了对转录调控网络的深入理解。
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以上文献均涉及Cyclin T1抗体的应用，涵盖病毒学、肿瘤学及基础转录机制研究。
Cyclin T1 is a regulatory subunit of the cyclin-dependent kinase 9 (CDK9), forming the positive transcription elongation factor b (P-TEFb) complex. This complex is essential for transcriptional elongation by phosphorylating RNA polymerase II, enabling the transition from paused transcription to productive elongation. Cyclin T1 is particularly notable for its role in HIV-1 replication, where it interacts with the viral Tat protein to enhance viral transcription. Dysregulation of Cyclin T1 has been implicated in cancer, neurological disorders, and inflammatory diseases, making it a focal point in molecular and clinical research.
Cyclin T1 antibodies are crucial tools for studying its expression, localization, and interactions in cellular pathways. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to explore Cyclin T1's involvement in cell cycle regulation, viral pathogenesis, and transcriptional control. Specific antibodies can distinguish Cyclin T1 from other cyclin family members, aiding in mechanistic studies of P-TEFb function. Additionally, they are employed in disease models to assess Cyclin T1's role in tumor progression or HIV latency reversal. Validated antibodies with high specificity are vital for accurate detection, supporting both basic research and therapeutic development targeting transcriptional dysregulation.
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