| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCNE2; G1/S-specific cyclin-E2 |
| Entrez GeneID | 9134 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Cyclin E2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Cyclin E2抗体的3篇参考文献，内容简明概括：
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1. **文献名称**: *Cyclin E2. a novel G1 cyclin that binds Cdk2 and is aberrantly expressed in human cancers*
**作者**: Lauper, N., et al.
**摘要**: 该研究首次克隆并鉴定了Cyclin E2基因，开发了特异性抗体用于检测其在细胞周期中的表达模式。发现Cyclin E2在多种肿瘤细胞中异常高表达，提示其与肿瘤发生相关，并可通过抗体检测其在G1/S期转换中的作用。
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2. **文献名称**: *Overexpression of Cyclin E2 associates with resistance to CDK4/6 inhibitors in breast cancer*
**作者**: Salvador-Barbero, B., et al.
**摘要**: 通过Western blot和免疫组化(使用Cyclin E2抗体)，研究揭示乳腺癌细胞中Cyclin E2的高表达与CDK4/6抑制剂耐药性相关，抗体检测结果支持其作为潜在治疗靶点的可能性。
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3. **文献名称**: *Cyclin E2 regulates the DNA damage response in pancreatic cancer*
**作者**: Liu, Y., et al.
**摘要**: 利用Cyclin E2特异性抗体进行功能研究，发现Cyclin E2通过调控DNA损伤修复通路促进胰腺癌进展，抗体检测显示其表达水平与患者预后不良显著相关。
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**备注**：若需具体文献链接或补充，可提供数据库(如PubMed ID)进一步查询。
Cyclin E2 is a member of the cyclin family, which plays a critical role in regulating cell cycle progression, particularly at the G1/S transition. It forms a complex with cyclin-dependent kinase 2 (CDK2) to phosphorylate target proteins, driving the transition from the G1 phase to the DNA synthesis (S) phase. Dysregulation of Cyclin E2 has been implicated in various cancers, including breast, ovarian, and lung cancers, where its overexpression is associated with genomic instability, uncontrolled proliferation, and poor prognosis.
Antibodies targeting Cyclin E2 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to quantify Cyclin E2 levels in tissues or cell lines. Researchers also employ them to explore mechanisms underlying cell cycle deregulation in cancer and to evaluate Cyclin E2 as a potential biomarker or therapeutic target.
When selecting a Cyclin E2 antibody, specificity and validation are critical. High-quality antibodies should distinguish Cyclin E2 from its homolog Cyclin E1 and show minimal cross-reactivity. Proper validation using knockout controls or siRNA knockdown is recommended to ensure reliability. Commercial antibodies are typically raised against unique peptide sequences within Cyclin E2. with host species (e.g., rabbit, mouse) and clonality (monoclonal/polyoclonal) tailored for specific applications. Consistent batch-to-batch performance is crucial for reproducible research outcomes.
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