首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肿瘤坏死因子配体相关分子-1(TL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFSF15含量。TNFSF15，也称为TL1A，是一种肿瘤坏死因子超家族成员，主要在免疫细胞中表达。其通过与DR3受体结合激活信号通路，参与炎症反应、细胞凋亡和肿瘤血管生成。研究显示，TNFSF15在多种疾病如炎症性肠病和肿瘤中扮演重要角色。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研实验中TL1蛋白表达水平的动态监测，例如炎症机制研究、免疫调控模型构建或药物干预效果评估等领域。通过标准曲线定量分析，研究者可精确测定不同生理或病理状态下样本中TL1的浓度变化，为探索该分子在免疫相关疾病中的作用提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnfsf15 ELISA Kit; Tl1 ELISA Kit; Vegi ELISA Kit; Tumor necrosis factor ligand superfamily member 15 ELISA Kit; TNF ligand-related molecule 1 ELISA Kit; Vascular endothelial cell growth inhibitor ELISA Kit
缩写：TNFSF15
Uniprot No.：Q5UBV8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-108 | |||||
回收率：
| The recovery of mouse TL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-93 | ||||
| EDTA plasma (n=4) | 94 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.8712.6412.7562.601 20002.0862.0672.0771.922 10001.3481.3251.3371.182 5000.8110.8260.8190.664 2500.5050.5440.5250.370 1250.2930.2990.2960.141 62.50.2190.2350.2270.072 00.1540.1560.155 | ||||||
货期：3-5 working days
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