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产品描述：人肿瘤坏死因子超家族15(TL1A/TNFSF15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TNFSF15含量。TNFSF15 是一个备受关注的靶点。它在免疫系统等生理过程中扮演重要角色。研究机制方面，其可能通过与特定受体结合，调控细胞的生长、分化和凋亡等，在炎症、肿瘤等疾病的发生发展中起到一定作用，为相关疾病治疗提供潜在方向。试剂盒检测范围为0.78 ng/mL-50 ng/mL，本产品在炎症机制研究、药物靶点验证、生物标志物筛选等方向具有重要应用价值，为探索TL1A在免疫调控网络中的作用提供了高灵敏度的检测方案，实验数据可为相关领域的分子机制研究提供可靠依据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNFSF15; TL1; VEGI; Tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; Vascular endothelial cell growth inhibitor
缩写：TNFSF15
Uniprot No.：O95150
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TL1A/TNFSF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-93 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 86-97 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 89-105 | ||||||
回收率：
| The recovery of human TL1A/TNFSF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 92-106 | |||||
| EDTA plasma (n=4) | 101 | 95-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 502.4712.3972.4342.295 251.8901.7921.8411.702 12.51.2591.2231.2411.102 6.250.8410.8320.8370.698 3.120.4690.4550.4620.323 1.560.3240.3140.3190.180 0.780.2290.2180.2240.085 00.1410.1370.139 | |||||||
货期：3-5 working days
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