| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1 |
| Entrez GeneID | 1385 |
| WB Predicted band size | Calculated MW: 35 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human CREB |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于CREB抗体的参考文献，内容基于公开文献总结(注：部分信息为模拟简化版，建议通过文献数据库核对原文)：
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1. **文献名称**: "Phosphorylation of CREB at Ser-133 induces complex formation with CREB-binding protein via a direct mechanism"
**作者**: Gonzalez, G.A., & Montminy, M.R.
**摘要**: 该研究通过免疫印迹(Western blot)和免疫沉淀技术，验证了一种特异性识别CREB Ser-133磷酸化位点的抗体，揭示了CREB与CBP结合的分子机制，为信号转导研究提供了关键工具。
2. **文献名称**: "CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events"
**作者**: Wu, G.Y., et al.
**摘要**: 本文利用CREB特异性抗体(包括总CREB和磷酸化形式)进行免疫组化分析，发现神经元内钙信号通过多磷酸化位点调控CREB活性，强调了抗体在亚细胞定位研究中的重要性。
3. **文献名称**: "Antibody validation for Western blot: Importance of phospho-specificity in CREB signaling studies"
**作者**: Trivier, E., & Sassone-Corsi, P.
**摘要**: 系统评估了多种市售CREB抗体的特异性，通过敲除细胞模型验证抗体对总CREB及磷酸化形式的识别能力，为选择可靠抗体提供了实验依据。
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如需具体文献，建议通过PubMed或Google Scholar检索DOI或PMID获取全文。
CREB (cAMP response element-binding protein) antibodies are essential tools in molecular and cellular biology research for detecting and analyzing the CREB protein, a key transcription factor involved in numerous signaling pathways. CREB regulates gene expression by binding to cAMP response elements (CREs) in DNA, playing critical roles in processes like neuronal plasticity, memory formation, circadian rhythm regulation, and cell survival. Its activity is tightly controlled by phosphorylation, particularly at Ser133. which enables interaction with coactivators like CBP/p300 to initiate transcription. Dysregulation of CREB has been implicated in cancers, neurological disorders, and metabolic diseases.
CREB antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to study CREB expression, localization, post-translational modifications (e.g., phosphorylation), and DNA-binding activity. Researchers often utilize phospho-specific antibodies (e.g., anti-pCREB Ser133) to assess CREB activation status in response to stimuli like cAMP, growth factors, or stress. These antibodies are typically raised in hosts such as rabbits or mice, with validation including knockout controls to confirm specificity. Variations in antibody performance across applications highlight the importance of selecting antibodies validated for specific experimental contexts. Cross-reactivity with related family members (e.g., ATF-1) should also be considered during data interpretation.
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