| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | COX5B; Cytochrome c oxidase subunit 5B; mitochondrial; Cytochrome c oxidase polypeptide Vb |
| Entrez GeneID | 1329 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human COX5B |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与COX5B抗体相关的模拟参考文献(基于研究领域常见主题的合理推测，非真实文献)：
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1. **文献名称**: *COX5B expression correlates with mitochondrial dysfunction in colorectal cancer progression*
**作者**: Li, X. et al.
**摘要**: 本研究通过免疫组化(IHC)和Western blot(WB)分析COX5B在结直肠癌组织中的表达，发现COX5B蛋白水平降低与患者预后不良相关，且其缺失通过抑制线粒体呼吸链复合体IV活性促进癌细胞转移。
2. **文献名称**: *COX5B antibody reveals its role in modulating HIF-1α stability under hypoxia*
**作者**: Martinez, R. & Kim, S.
**摘要**: 研究利用COX5B特异性抗体证实，缺氧条件下COX5B与HIF-1α的相互作用通过调控线粒体ROS生成影响HIF-1α稳定性，揭示了其在肿瘤代谢适应中的新机制。
3. **文献名称**: *Loss of COX5B impairs synaptic function in Alzheimer’s disease models*
**作者**: Chen, L. et al.
**摘要**: 通过免疫荧光和COX5B抗体检测，发现阿尔茨海默病小鼠模型中COX5B蛋白表达下降，导致神经元线粒体能量代谢障碍和突触可塑性受损，提示其与神经退行性病变相关。
4. **文献名称**: *COX5B regulates skeletal muscle regeneration via mitochondrial dynamics*
**作者**: Park, J.H. et al.
**摘要**: 使用COX5B抗体进行蛋白质定位分析，发现其在肌肉干细胞分化中通过调控线粒体融合-分裂平衡影响成肌细胞增殖与分化，为肌肉再生障碍提供了潜在治疗靶点。
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**注**：以上文献为模拟内容，实际研究中请通过PubMed或Web of Science以“COX5B antibody”及关键词(如mitochondria、cancer、neurodegeneration)检索真实文献。
The COX5B antibody is a research tool designed to detect cytochrome c oxidase subunit 5B (COX5B), a nuclear-encoded subunit of mitochondrial complex IV (cytochrome c oxidase), which is the terminal enzyme in the mitochondrial electron transport chain (ETC). COX5B plays a critical role in modulating the activity and stability of complex IV, essential for aerobic ATP production. Dysregulation of COX5B has been linked to mitochondrial disorders, cancer, and neurodegenerative diseases.
COX5B antibodies are widely used in immunoblotting (Western blot), immunohistochemistry (IHC), and immunofluorescence (IF) to study protein expression, subcellular localization, and mitochondrial function in various tissues or cell types. These antibodies help investigate COX5B's role in oxidative phosphorylation, cellular metabolism, and apoptosis. Researchers also utilize them to explore correlations between COX5B expression levels and diseases, such as tumor progression or metabolic syndromes.
Validated COX5B antibodies exhibit specificity for human, mouse, or rat samples, often confirmed by knockout controls. Commercial antibodies may vary in host species (e.g., rabbit, mouse) and conjugates (e.g., HRP, FITC). Proper validation ensures reliability in detecting both endogenous and overexpressed COX5B in experimental models, supporting studies in cell biology, cancer research, and mitochondrial medicine.
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