| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UFC1 |
| Uniprot No | Q9Y3C8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-167aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMADEATRRVVSEIPVLKTNAGPRDRELWVQ RLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKCWYIHDLLKYEF DIEFDIPITYPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVP KFGLAHLMALGLGPWLAVEIPDLIQKGVIQHKEKCNQ |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UFC1重组蛋白的3篇参考文献示例(注：UFC1相关研究较少，以下为模拟内容，实际文献需通过学术数据库验证)：
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1. **文献名称**: *Cloning and expression of UFC1 recombinant protein in E. coli*
**作者**: Zhang L, et al.
**摘要**: 本研究成功构建了UFC1基因的原核表达载体，并利用大肠杆菌系统高效表达可溶性UFC1重组蛋白。通过His标签亲和层析纯化获得高纯度蛋白，为后续泛素化机制研究提供材料基础。
2. **文献名称**: *Structural analysis of UFC1 in the ubiquitin-conjugating enzyme complex*
**作者**: Thompson R, et al.
**摘要**: 通过X射线晶体学解析UFC1重组蛋白的结构，发现其与UBC12形成复合物，关键结构域参与催化泛素转移，揭示了其在蛋白质降解通路中的分子机制。
3. **文献名称**: *Functional characterization of UFC1 mutations in cancer cell lines*
**作者**: Chen H, et al.
**摘要**: 利用重组UFC1蛋白进行体外功能实验，发现其某些突变体导致泛素化活性丧失，并证实UFC1异常表达与肿瘤细胞增殖及化疗耐药性相关。
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如需具体文献，建议在PubMed或Web of Science以关键词“UFC1 recombinant”“Ubiquitin-fold conjugating enzyme 1”检索，并筛选近年高被引研究。
**Background of UFC1 Recombinant Protein**
UFC1 (Ubiquitin-Fold Conjugating Enzyme 1), also known as Ubiquitin Fusion Conjugation 1. is a critical component of the ubiquitin-proteasome system (UPS), a major pathway for regulated protein degradation in eukaryotic cells. This small, highly conserved protein plays a role in the conjugation of ubiquitin-like modifiers to target proteins, a process essential for maintaining cellular homeostasis, DNA repair, and stress response. UFC1 is structurally characterized by a ubiquitin-like (UBL) domain, enabling it to interact with other enzymes in the ubiquitination cascade, such as E1 activating enzymes and E3 ligases.
Recombinant UFC1 protein is produced using biotechnological methods, typically through expression in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., yeast or mammalian cells). The recombinant form retains the functional properties of native UFC1. including its ability to participate in ubiquitin transfer reactions. Researchers utilize UFC1 recombinant protein to study UPS mechanisms, particularly in vitro assays exploring ubiquitination dynamics, substrate recognition, and enzyme kinetics. Its applications extend to investigating diseases linked to UPS dysregulation, such as cancer, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s), and autoimmune conditions.
Additionally, UFC1 serves as a tool for drug discovery, aiding in the identification of inhibitors or modulators targeting the ubiquitination pathway. The production of recombinant UFC1 ensures high purity and consistency, addressing challenges associated with isolating the protein from native tissues. Ongoing research continues to unveil its regulatory roles in cell cycle control, apoptosis, and response to proteotoxic stress, solidifying its importance in both basic and translational biomedical studies.
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