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产品描述：人肿瘤坏死因子配体超家族成员25(TNFSF25)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFRSF25含量。TNFRSF25是一个靶点。其它在免疫调节等生理过程里有重要作用。研究机制上，主要是通过与配体结合来激活下游信号通路，进而参与调节T细胞活性、细胞存活等，有望为免疫相关疾病治疗提供新靶点。试剂盒检测范围为0.24 ng/mL-15 ng/mL，科研人员可通过该产品探索TNFSF25在自身免疫性疾病模型、肿瘤微环境调控或感染性疾病中的功能机制，尤其适合用于评估免疫细胞信号通路、炎症因子网络互作等基础研究；满足体外定量分析需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apo 3 ELISA Kit; Apo-3 ELISA Kit; Apo3 ELISA Kit; Apoptosis inducing receptor AIR ELISA Kit; Apoptosis inducing receptor ELISA Kit; Apoptosis mediating receptor ELISA Kit; Apoptosis mediating receptor DR 3 ELISA Kit; Apoptosis mediating receptor DR3 ELISA Kit; Apoptosis mediating receptor TRAMP ELISA Kit; Apoptosis-inducing receptor AIR ELISA Kit; Apoptosis-mediating receptor DR3 ELISA Kit; Apoptosis-mediating receptor TRAMP ELISA Kit; DDR 3 ELISA Kit; DDR3 ELISA Kit; Death domain receptor 3 ELISA Kit; Death domain receptor 3 soluble form ELISA Kit; Death receptor 3 ELISA Kit; Death receptor beta ELISA Kit; DR 3 ELISA Kit; DR3 ELISA Kit; LARD ELISA Kit; Lymphocyte associated receptor of death ELISA Kit; Lymphocyte-associated receptor of death ELISA Kit; Protein WSL ELISA Kit; Protein WSL-1 ELISA Kit; TNF receptor superfamily member 25 ELISA Kit; TNFR25 ELISA Kit; TNFRSF 12 ELISA Kit; TNFRSF 25 ELISA Kit; TNFRSF12 ELISA Kit; TNFRSF12, formerly ELISA Kit; TNFRSF25 ELISA Kit; TNR25_HUMAN ELISA Kit; TR 3 ELISA Kit; TR3 ELISA Kit; TRAMP ELISA Kit; Translocating chain association membrane protein ELISA Kit; Tumor necrosis factor receptor superfamily member 12 ELISA Kit; Tumor necrosis factor receptor superfamily member 25 ELISA Kit; Tumor necrosis factor receptor superfamily, member 12 (translocating chain association membrane protein) ELISA Kit; Tumor necrosis factor receptor superfamily, member 12, formerly ELISA Kit; WSL 1 ELISA Kit; WSL ELISA Kit; WSL LR ELISA Kit; WSL protein ELISA Kit; WSL1 ELISA Kit; WSL1 protein ELISA Kit; WSLLR ELISA Kit
缩写：TNFRSF25
Uniprot No.：Q93038
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.24 ng/mL-15 ng/mL
灵敏度：0.06 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 85-93 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-93 | |||||
回收率：
| The recovery of human TNFRSF25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 83 | 80-86 | ||||
| EDTA plasma (n=4) | 91 | 88-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected152.4642.4982.4812.3217.51.6961.6681.6821.5223.751.1011.1351.1180.9581.880.7120.7320.7220.5620.940.5130.5360.5250.3650.470.3980.3870.3930.2330.240.2970.2760.2870.12700.1610.1590.160 | ||||||
货期：3-5 working days
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