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Mouse Tumor necrosis factor receptor superfamily member 17(TNFRSF17) ELISA kit

  • 中文名: 小鼠肿瘤坏死因子配体超家族成员17(TNFSF17)酶联免疫试剂盒
货号: IPD-EL023974MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠肿瘤坏死因子配体超家族成员17(TNFSF17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的TNFRSF17含量。TNFRSF17 是一个重要靶点。它属肿瘤坏死因子受体超家族成员,在B淋巴细胞发育与功能维持中起关键作用。研究聚焦其信号传导机制,探索与免疫疾病、血液系统肿瘤关联,有望为相关疾病诊断和治疗提供新思路。试剂盒检测范围为0.39 ng/ml- 25 ng/ml.,支持科研人员高效开展小鼠模型中的免疫调节研究、肿瘤微环境分析或炎症性疾病机制探索,尤其适用于评估实验动物体内TNFSF17表达水平变化与特定病理状态的相关性,为免疫学、分子生物学及药理学研究提供可靠的数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Tnfrsf17; Bcm; Bcma; Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD antigen CD269
  • 缩写:
    TNFRSF17
  • Uniprot No.:
    O88472
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.39 ng/ml - 25 ng/ml.
  • 灵敏度:
    0.1 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFRSF17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    99


    Range %

    90-104


    1:2

    Average %

    100


    Range %

    90-105


    1:4

    Average %

    96


    Range %

    89-100


    1:8

    Average %

    93


    Range %

    89-97


  • 回收率:

    The recovery of mouse TNFRSF17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    88-97


    EDTA plasma (n=4)

    102

    95-107


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    25

    2.606

    2.567

    2.587

    2.410


    12.5

    1.832

    1.795

    1.814

    1.637


    6.25

    1.229

    1.200

    1.215

    1.038


    3.125

    0.708

    0.688

    0.698

    0.521


    1.56

    0.425

    0.416

    0.421

    0.244


    0.78

    0.303

    0.289

    0.296

    0.119


    0.39

    0.229

    0.232

    0.231

    0.054


    0

    0.179

    0.175

    0.177




  • 货期:
    3-5 working days

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